國立虎尾科技大學 |

Thermodynamics of Adsorption of GABA onto Lipid Vesicles Containing DMPG and DMPC Using Isothermal Titration Calorimetry.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Thermodynamics of Adsorption of GABA onto Lipid Vesicles Containing DMPG and DMPC Using Isothermal Titration Calorimetry./
Author:	Tian, Dayong.
Description:	1 online resource (139 pages)
Notes:	Source: Masters Abstracts International, Volume: 57-02.
Contained By:	Masters Abstracts International57-02(E).
Subject:	Chemistry. -
Online resource:	click for full text (PQDT)
ISBN:	9780355409093

Thermodynamics of Adsorption of GABA onto Lipid Vesicles Containing DMPG and DMPC Using Isothermal Titration Calorimetry.
Tian, Dayong.

Thermodynamics of Adsorption of GABA onto Lipid Vesicles Containing DMPG and DMPC Using Isothermal Titration Calorimetry. - 1 online resource (139 pages)

Source: Masters Abstracts International, Volume: 57-02.

Thesis (M.S.)--Dartmouth College, 2017.

Includes bibliographical references

During a synaptic transmission, the pre-synaptic neuron releases neurotransmitters into the synapse, where they bind to post-synaptic receptors specific to them, thereby propagating the signal. However, the non-specific adsorption of neurotransmitter molecules to lipid membranes has also been speculated to impact synaptic transmission. Though the clearance of adsorbed metabolic wastes has been suggested as an underlying vital function of sleep, a process conserved for all animals, the adsorption of neurotransmitters to lipid vesicles has rarely been quantitatively described. Thermodynamic measurements of adsorption for the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) to model lipid membranes composed of 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DMPG) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) are presented for the first time in this study.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355409093Subjects--Topical Terms:

593913
Chemistry.
Index Terms--Genre/Form:

554714
Electronic books.

Thermodynamics of Adsorption of GABA onto Lipid Vesicles Containing DMPG and DMPC Using Isothermal Titration Calorimetry.
LDR:03206ntm a2200373Ki 4500 001 918863
005 20181106104113.5
006 m o u
007 cr mn||||a|a||
008 190606s2017 xx obm 000 0 eng d
020 $a 9780355409093
035 $a (MiAaPQ)AAI10691121
035 $a (MiAaPQ)dartmouth:11272
035 $a AAI10691121
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Tian, Dayong. $3 1193304
245 1 0 $a Thermodynamics of Adsorption of GABA onto Lipid Vesicles Containing DMPG and DMPC Using Isothermal Titration Calorimetry.
264 0 $c 2017
300 $a 1 online resource (139 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 57-02.
500 $a Adviser: Robert S. Cantor.
502 $a Thesis (M.S.)--Dartmouth College, 2017.
504 $a Includes bibliographical references
520 $a During a synaptic transmission, the pre-synaptic neuron releases neurotransmitters into the synapse, where they bind to post-synaptic receptors specific to them, thereby propagating the signal. However, the non-specific adsorption of neurotransmitter molecules to lipid membranes has also been speculated to impact synaptic transmission. Though the clearance of adsorbed metabolic wastes has been suggested as an underlying vital function of sleep, a process conserved for all animals, the adsorption of neurotransmitters to lipid vesicles has rarely been quantitatively described. Thermodynamic measurements of adsorption for the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) to model lipid membranes composed of 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DMPG) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) are presented for the first time in this study.
520 $a Lipids of varying lipid composition and concentration were prepared using an extruder. Isothermal titration calorimetry (ITC) was used alongside fitting models to derive thermodynamic parameters, including adsorption affinity, Kads. ITC results were obtained for the adsorption of GABA to 1:1 mixtures of DMPG and DMPC, with consistent fitted K ads values in the order of magnitude of 101 to 103 M--1. The size distribution of extruded lipids was characterized using dynamic light scattering (DLS), and ITC results were further supported with techniques such as concatenation and custom.
520 $a The system explored is physiologically significant. GABA is the most common inhibitory neurotransmitter, and neuronal membranes contain a high fraction (upwards of 20%) of anionic lipids. Consistent Kads values, obtained using ITC, for the affinity of neurotransmitters to receptor-free model lipids, suggests that adsorption is occurring as a non-specific interaction, shedding further insight towards the purpose of sleep.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Chemistry. $3 593913
650 4 $a Biophysics. $3 581576
650 4 $a Thermodynamics. $3 596513
655 7 $a Electronic books. $2 local $3 554714
690 $a 0485
690 $a 0786
690 $a 0348
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Dartmouth College. $b Chemistry. $3 1192688
773 0 $t Masters Abstracts International $g 57-02(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10691121 $z click for full text (PQDT)