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New Insights into the Assembly Mechanism of an RNA Polymerase III-Specific Transcription Complex on a Drosophila U6 snRNA Gene Promoter.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	New Insights into the Assembly Mechanism of an RNA Polymerase III-Specific Transcription Complex on a Drosophila U6 snRNA Gene Promoter./
Author:	Moreno, Ann Marie.
Description:	1 online resource (133 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Contained By:	Dissertation Abstracts International79-10B(E).
Subject:	Biology. -
Online resource:	click for full text (PQDT)
ISBN:	9780355985047

New Insights into the Assembly Mechanism of an RNA Polymerase III-Specific Transcription Complex on a Drosophila U6 snRNA Gene Promoter.
Moreno, Ann Marie.

New Insights into the Assembly Mechanism of an RNA Polymerase III-Specific Transcription Complex on a Drosophila U6 snRNA Gene Promoter. - 1 online resource (133 pages)

Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.

Thesis (Ph.D.)--University of California, San Diego, 2018.

Includes bibliographical references

In metazoans, the genes that code for the U6 small nuclear RNA (snRNA) have promoters that consist of a proximal sequence element (PSE) and a TATA box. The PSE is recognized by the multi-subunit small nuclear RNA activating protein complex (SNAPc). SNAPc forms a complex on the U6 promoter with TFIIIB, an RNA polymerase III-specific general transcription factor that includes TBP, Brf1 (Brf2 in vertebrates), and Bdp1. Here we show that, in the fruit fly Drosophila melanogaster, DmSNAPc directly recruits Bdp1 to the U6 promoter. We also demonstrate that an 87-residue region of Bdp1 is sufficient for this recruitment and is, furthermore, sufficient to recruit TBP to the TATA box. The non-conserved N-terminal tail of TBP also plays a role in stabilizing TBP incorporation into the DmSNAPc-Bdp1 complex. While Bdp1 recruitment by DmSNAPc is independent of the presence of TBP, Brf1, or a TATA box, it does require that DmSNAPc be bound to a U6 gene PSE rather than a PSE derived from a U1 gene (which is transcribed by RNA polymerase II). We also confirmed that Brf1 is present at U6 snRNA genes but not at U1 genes. These findings further develop the concept that DmSNAPc adopts different conformations upon binding U6 and U1 gene PSEs, and that these different DmSNAPc conformations lead to the subsequent recruitment of distinct general transcription factors and RNA polymerases for U6 and U1 gene transcription.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355985047Subjects--Topical Terms:

599573
Biology.
Index Terms--Genre/Form:

554714
Electronic books.

New Insights into the Assembly Mechanism of an RNA Polymerase III-Specific Transcription Complex on a Drosophila U6 snRNA Gene Promoter.
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245 1 0 $a New Insights into the Assembly Mechanism of an RNA Polymerase III-Specific Transcription Complex on a Drosophila U6 snRNA Gene Promoter.
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500 $a Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
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502 $a Thesis (Ph.D.)--University of California, San Diego, 2018.
504 $a Includes bibliographical references
520 $a In metazoans, the genes that code for the U6 small nuclear RNA (snRNA) have promoters that consist of a proximal sequence element (PSE) and a TATA box. The PSE is recognized by the multi-subunit small nuclear RNA activating protein complex (SNAPc). SNAPc forms a complex on the U6 promoter with TFIIIB, an RNA polymerase III-specific general transcription factor that includes TBP, Brf1 (Brf2 in vertebrates), and Bdp1. Here we show that, in the fruit fly Drosophila melanogaster, DmSNAPc directly recruits Bdp1 to the U6 promoter. We also demonstrate that an 87-residue region of Bdp1 is sufficient for this recruitment and is, furthermore, sufficient to recruit TBP to the TATA box. The non-conserved N-terminal tail of TBP also plays a role in stabilizing TBP incorporation into the DmSNAPc-Bdp1 complex. While Bdp1 recruitment by DmSNAPc is independent of the presence of TBP, Brf1, or a TATA box, it does require that DmSNAPc be bound to a U6 gene PSE rather than a PSE derived from a U1 gene (which is transcribed by RNA polymerase II). We also confirmed that Brf1 is present at U6 snRNA genes but not at U1 genes. These findings further develop the concept that DmSNAPc adopts different conformations upon binding U6 and U1 gene PSEs, and that these different DmSNAPc conformations lead to the subsequent recruitment of distinct general transcription factors and RNA polymerases for U6 and U1 gene transcription.
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