語系:
繁體中文
English
說明(常見問題)
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Recombinant Enzymes - From Basic Sci...
~
Amid, Azura.
Recombinant Enzymes - From Basic Science to Commercialization
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Recombinant Enzymes - From Basic Science to Commercialization/ edited by Azura Amid.
其他作者:
Amid, Azura.
面頁冊數:
X, 188 p. 61 illus.online resource. :
Contained By:
Springer Nature eBook
標題:
Biology—Technique. -
電子資源:
https://doi.org/10.1007/978-3-319-12397-4
ISBN:
9783319123974
Recombinant Enzymes - From Basic Science to Commercialization
Recombinant Enzymes - From Basic Science to Commercialization
[electronic resource] /edited by Azura Amid. - 1st ed. 2015. - X, 188 p. 61 illus.online resource.
Introduction to Recombinant Enzyme Pre-commercialization -- Recombinant Enzyme: Cloning and Expression -- Common Laboratory Procedure -- Characterization of Recombinant Enzymes -- Purification of Recombinant Protein for Industrial Use -- Recombinant Enzyme Fermentation -- Scaling-up Recombinant Enzyme Fermentation -- Downstream Processing of Recombinant Enzymes for Commercialization -- Economic and Environmental Evaluation of Recombinant Enzyme Production -- Case Study: Recombinant Bromelain Selection -- Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes -- Case Study: Recombinant Bromelain Downstream Processing.
This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product. Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host. A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production. This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization. .
ISBN: 9783319123974
Standard No.: 10.1007/978-3-319-12397-4doiSubjects--Topical Terms:
1256859
Biology—Technique.
LC Class. No.: QH324
Dewey Class. No.: 570.28
Recombinant Enzymes - From Basic Science to Commercialization
LDR
:03558nam a22004095i 4500
001
962046
003
DE-He213
005
20200704232654.0
007
cr nn 008mamaa
008
201211s2015 gw | s |||| 0|eng d
020
$a
9783319123974
$9
978-3-319-12397-4
024
7
$a
10.1007/978-3-319-12397-4
$2
doi
035
$a
978-3-319-12397-4
050
4
$a
QH324
072
7
$a
PS
$2
bicssc
072
7
$a
SCI008000
$2
bisacsh
072
7
$a
PS
$2
thema
072
7
$a
PDN
$2
thema
082
0 4
$a
570.28
$2
23
245
1 0
$a
Recombinant Enzymes - From Basic Science to Commercialization
$h
[electronic resource] /
$c
edited by Azura Amid.
250
$a
1st ed. 2015.
264
1
$a
Cham :
$b
Springer International Publishing :
$b
Imprint: Springer,
$c
2015.
300
$a
X, 188 p. 61 illus.
$b
online resource.
336
$a
text
$b
txt
$2
rdacontent
337
$a
computer
$b
c
$2
rdamedia
338
$a
online resource
$b
cr
$2
rdacarrier
347
$a
text file
$b
PDF
$2
rda
505
0
$a
Introduction to Recombinant Enzyme Pre-commercialization -- Recombinant Enzyme: Cloning and Expression -- Common Laboratory Procedure -- Characterization of Recombinant Enzymes -- Purification of Recombinant Protein for Industrial Use -- Recombinant Enzyme Fermentation -- Scaling-up Recombinant Enzyme Fermentation -- Downstream Processing of Recombinant Enzymes for Commercialization -- Economic and Environmental Evaluation of Recombinant Enzyme Production -- Case Study: Recombinant Bromelain Selection -- Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes -- Case Study: Recombinant Bromelain Downstream Processing.
520
$a
This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product. Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host. A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production. This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization. .
650
0
$a
Biology—Technique.
$3
1256859
650
0
$a
Industrial engineering.
$3
679492
650
0
$a
Production engineering.
$3
566269
650
0
$a
Medicine.
$3
644133
650
1 4
$a
Biological Techniques.
$3
678565
650
2 4
$a
Industrial and Production Engineering.
$3
593943
650
2 4
$a
Biomedicine, general.
$3
1253757
700
1
$a
Amid, Azura.
$4
edt
$4
http://id.loc.gov/vocabulary/relators/edt
$3
1065484
710
2
$a
SpringerLink (Online service)
$3
593884
773
0
$t
Springer Nature eBook
776
0 8
$i
Printed edition:
$z
9783319123981
776
0 8
$i
Printed edition:
$z
9783319123967
776
0 8
$i
Printed edition:
$z
9783319356051
856
4 0
$u
https://doi.org/10.1007/978-3-319-12397-4
912
$a
ZDB-2-SBL
912
$a
ZDB-2-SXB
950
$a
Biomedical and Life Sciences (SpringerNature-11642)
950
$a
Biomedical and Life Sciences (R0) (SpringerNature-43708)
筆 0 讀者評論
多媒體
評論
新增評論
分享你的心得
Export
取書館別
處理中
...
變更密碼[密碼必須為2種組合(英文和數字)及長度為10碼以上]
登入