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Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases/ by Matthew Jenner.
作者:	Jenner, Matthew.
面頁冊數:	XVIII, 176 p. 136 illus., 99 illus. in color.online resource. :
Contained By:	Springer Nature eBook
標題:	Mass spectrometry. -
電子資源:	https://doi.org/10.1007/978-3-319-32723-5
ISBN:	9783319327235

Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases
Jenner, Matthew.

Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases[electronic resource] /by Matthew Jenner. - 1st ed. 2016. - XVIII, 176 p. 136 illus., 99 illus. in color.online resource. - Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053. - Springer Theses, Recognizing Outstanding Ph.D. Research,.

Introduction -- Materials and Methods -- Substrate Specificity of Ketosynthase Domains Part I: β-Branched Acyl Chains -- Substrate Specificity of Ketosynthase Domains Part II: Amino Acid-Containing Acyl Chains -- Synthesis of Acyl-Acyl Carrier Proteins and their use in Studying Polyketide Synthase Enzymology -- Substrate Specificity of Ketosynthase Domains Part III: Elongation-Based Substrate Specificity.

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully studied the specificity of a range of KS domains from the bacillaene and psymberin PKSs with regard to the initial acylation step of KS-catalysis. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering. The documentation of this research is a useful reference and guideline for students starting a PhD in this field.

ISBN: 9783319327235

Standard No.: 10.1007/978-3-319-32723-5doiSubjects--Topical Terms:

583253
Mass spectrometry.

LC Class. No.: QD96.M3

Dewey Class. No.: 543.65

Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases
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