國立虎尾科技大學 |

Advancing Development of Synthetic Gene Regulators = With the Power of High-Throughput Sequencing in Chemical Biology /

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Advancing Development of Synthetic Gene Regulators/ by Anandhakumar Chandran.
其他題名:	With the Power of High-Throughput Sequencing in Chemical Biology /
作者:	Chandran, Anandhakumar.
面頁冊數:	XV, 114 p. 49 illus., 44 illus. in color.online resource. :
Contained By:	Springer Nature eBook
標題:	Biotechnology. -
電子資源:	https://doi.org/10.1007/978-981-10-6547-7
ISBN:	9789811065477

Advancing Development of Synthetic Gene Regulators = With the Power of High-Throughput Sequencing in Chemical Biology /
Chandran, Anandhakumar.

Advancing Development of Synthetic Gene RegulatorsWith the Power of High-Throughput Sequencing in Chemical Biology /[electronic resource] :by Anandhakumar Chandran. - 1st ed. 2018. - XV, 114 p. 49 illus., 44 illus. in color.online resource. - Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053. - Springer Theses, Recognizing Outstanding Ph.D. Research,.

Overview of Next-Generation Sequencing Technologies and its application in Chemical Biology -- Next Generation Sequencing Studies Guide the Design of Pyrrole-Imidazole Polyamides with Improved Binding Specificity by the Addition of β-alanine -- Genome-Wide Assessment of the Binding Effects of Artificial Transcriptional Activators by Utilizing the Power of High-Throughput Sequencing -- Deciphering the genomic targets of alkylating polyamide conjugates using high-throughput sequencing.

This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence. .

ISBN: 9789811065477

Standard No.: 10.1007/978-981-10-6547-7doiSubjects--Topical Terms:

554955
Biotechnology.

LC Class. No.: TP248.13-248.65

Dewey Class. No.: 660.6

Advancing Development of Synthetic Gene Regulators = With the Power of High-Throughput Sequencing in Chemical Biology /
LDR:03944nam a22004095i 4500 001 999110
003 DE-He213
005 20200706175932.0
007 cr nn 008mamaa
008 201225s2018 si | s |||| 0|eng d
020 $a 9789811065477 $9 978-981-10-6547-7
024 7 $a 10.1007/978-981-10-6547-7 $2 doi
035 $a 978-981-10-6547-7
050 4 $a TP248.13-248.65
072 7 $a TCB $2 bicssc
072 7 $a SCI010000 $2 bisacsh
072 7 $a TCB $2 thema
082 0 4 $a 660.6 $2 23
100 1 $a Chandran, Anandhakumar. $e author. $4 aut $4 http://id.loc.gov/vocabulary/relators/aut $3 1290677
245 1 0 $a Advancing Development of Synthetic Gene Regulators $h [electronic resource] : $b With the Power of High-Throughput Sequencing in Chemical Biology / $c by Anandhakumar Chandran.
250 $a 1st ed. 2018.
264 1 $a Singapore : $b Springer Singapore : $b Imprint: Springer, $c 2018.
300 $a XV, 114 p. 49 illus., 44 illus. in color. $b online resource.
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
347 $a text file $b PDF $2 rda
490 1 $a Springer Theses, Recognizing Outstanding Ph.D. Research, $x 2190-5053
505 0 $a Overview of Next-Generation Sequencing Technologies and its application in Chemical Biology -- Next Generation Sequencing Studies Guide the Design of Pyrrole-Imidazole Polyamides with Improved Binding Specificity by the Addition of β-alanine -- Genome-Wide Assessment of the Binding Effects of Artificial Transcriptional Activators by Utilizing the Power of High-Throughput Sequencing -- Deciphering the genomic targets of alkylating polyamide conjugates using high-throughput sequencing.
520 $a This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence. .
650 0 $a Biotechnology. $3 554955
650 0 $a Gene therapy. $3 582271
650 0 $a Bioorganic chemistry. $3 743635
650 2 4 $a Gene Therapy. $3 580629
650 2 4 $a Bioorganic Chemistry. $3 677511
710 2 $a SpringerLink (Online service) $3 593884
773 0 $t Springer Nature eBook
776 0 8 $i Printed edition: $z 9789811065460
776 0 8 $i Printed edition: $z 9789811065484
776 0 8 $i Printed edition: $z 9789811348990
830 0 $a Springer Theses, Recognizing Outstanding Ph.D. Research, $x 2190-5053 $3 1253569
856 4 0 $u https://doi.org/10.1007/978-981-10-6547-7
912 $a ZDB-2-CMS
912 $a ZDB-2-SXC
950 $a Chemistry and Materials Science (SpringerNature-11644)
950 $a Chemistry and Material Science (R0) (SpringerNature-43709)