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Single Step Purification of Human Pl...

Deal, Jacob R.

Single Step Purification of Human Plasma Fibronectin.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Single Step Purification of Human Plasma Fibronectin./
Author:	Deal, Jacob R.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	39 p.
Notes:	Source: Masters Abstracts International, Volume: 81-10.
Contained By:	Masters Abstracts International81-10.
Subject:	Chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27957679
ISBN:	9798641842325

Single Step Purification of Human Plasma Fibronectin.
Deal, Jacob R.

Single Step Purification of Human Plasma Fibronectin. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 39 p.

Source: Masters Abstracts International, Volume: 81-10.

Thesis (M.S.)--Northeastern University, 2020.

This item must not be sold to any third party vendors.

Fibronectin is a high molecular weight glycoprotein found throughout the extracellular matrix in humans. First purified in 1948 as a side product of a fibrinogen isolation, it was noted simply as cold-insoluble globulin. Following its independent discovery in fibroblast research, its abundance throughout the body, and its importance in regulating both disease and materials design, fibronectin has demanded the attention of researchers over the past five decades. With demand for the protein rising alongside its expanding utility, purification methods have been developed, iterated, and optimized to provide high quality material at higher yield and in fewer steps. Herein, the design and application of a modified one-step purification of fibronectin from human plasma is reported. Using gelatin affinity chromatography with a stepwise arginine gradient, the results from this study highlight a method for providing fibronectin from human plasma under non-denaturing conditions in less than one half the time currently used in the state-of-the art purification methods.

ISBN: 9798641842325Subjects--Topical Terms:

593913
Chemistry.
Subjects--Index Terms:

Cold-insoluble globulin

Single Step Purification of Human Plasma Fibronectin.
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020 $a 9798641842325
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100 1 $a Deal, Jacob R. $3 1335278
245 1 0 $a Single Step Purification of Human Plasma Fibronectin.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 39 p.
500 $a Source: Masters Abstracts International, Volume: 81-10.
500 $a Advisor: Deravi, Leila F.
502 $a Thesis (M.S.)--Northeastern University, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Fibronectin is a high molecular weight glycoprotein found throughout the extracellular matrix in humans. First purified in 1948 as a side product of a fibrinogen isolation, it was noted simply as cold-insoluble globulin. Following its independent discovery in fibroblast research, its abundance throughout the body, and its importance in regulating both disease and materials design, fibronectin has demanded the attention of researchers over the past five decades. With demand for the protein rising alongside its expanding utility, purification methods have been developed, iterated, and optimized to provide high quality material at higher yield and in fewer steps. Herein, the design and application of a modified one-step purification of fibronectin from human plasma is reported. Using gelatin affinity chromatography with a stepwise arginine gradient, the results from this study highlight a method for providing fibronectin from human plasma under non-denaturing conditions in less than one half the time currently used in the state-of-the art purification methods.
590 $a School code: 0160.
650 4 $a Chemistry. $3 593913
650 4 $a Biochemistry. $3 582831
653 $a Cold-insoluble globulin
653 $a Fibronectin
653 $a Fibronectin purification
653 $a Fibronectin storage
653 $a Protein purification
653 $a SFA
690 $a 0485
690 $a 0487
710 2 $a Northeastern University. $b Chemistry & Chemical Biology. $3 1335279
773 0 $t Masters Abstracts International $g 81-10.
790 $a 0160
791 $a M.S.
792 $a 2020
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27957679

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