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高壓加工處理對羽衣甘藍單穀胺酸葉酸含量及生物利用率的影響 = = Eff...

黃柏慈

高壓加工處理對羽衣甘藍單穀胺酸葉酸含量及生物利用率的影響 = = Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale /

Record Type:	Language materials, printed : Monograph/item
Title/Author:	高壓加工處理對羽衣甘藍單穀胺酸葉酸含量及生物利用率的影響 =/ 黃柏慈.
Reminder of title:	Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale /
remainder title:	Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale.
Author:	黃柏慈
Published:	雲林縣 :國立虎尾科技大學 , : 民113.07.,
Description:	[9], 104面 :圖, 表 ; : 30公分.;
Notes:	指導教授: 王鐘毅.
Subject:	mono/polyglutamate. -
Online resource:	電子資源

高壓加工處理對羽衣甘藍單穀胺酸葉酸含量及生物利用率的影響 = = Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale /
黃柏慈

高壓加工處理對羽衣甘藍單穀胺酸葉酸含量及生物利用率的影響 =Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale /Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale.黃柏慈. - 初版. - 雲林縣 :國立虎尾科技大學 ,民113.07. - [9], 104面 :圖, 表 ;30公分.

指導教授: 王鐘毅.

碩士論文--國立虎尾科技大學生物科技系碩士班.

含參考書目.

葉酸 ( Folate ) 是一種水溶性維生素，俗稱維生素 B9，是參與人體內許多重要生物反應的必須微量營養素。但人體無法自行合成葉酸，因此需透過每日的飲食或是營養保健品來補充，而綠色蔬菜是攝取天然葉酸的重要來源。一般成年人建議每日攝取 400 微克的葉酸，若長期攝取不足可能會增加罹患心血管疾病、癌症及神經管缺陷的風險。本研究利用高壓加工技術 ( High pressure processing, HPP ) 提升羽衣甘藍 ( Brassica oleracea var. sabellica ) 中天然葉酸的含量，並透過微生物法及高效液相層析法分析蔬菜中單穀胺酸及聚穀胺酸的含量。研究結果顯示，高壓導致蔬菜中的長鏈聚穀胺酸轉變為短鏈及單穀胺酸形式的葉酸。在不同壓力相同時間處理下 200 及 400 MPa 總葉酸含量為 237.06 及 236.32 μg/100 g，而 600 MPa 高壓處理 5 分鐘及 10 分鐘顯著提高羽衣甘藍中總葉酸的含量，分別由控制組的 219.64 μg/100 g 增加至 239.41 及 241.14 μg/100 g， 15 分鐘及 20 分鐘高壓處理總葉酸含量的變化較不顯著。此外，經熱處理後羽衣甘藍聚穀胺酸含量顯著提高、單穀胺酸含量顯著降低，而蔬菜冷凍 24 小時後雖然因解凍過程的水分流失導致總葉酸含量下降，卻也因冷凍使細胞損傷促使內源性酵素作用進而促進聚穀胺酸轉化為單穀胺酸。結果表示，葉酸具熱敏感性，可能會隨著溫度升高而產生降解。而高壓加工技術作為一種新興的非熱加工技術不僅可取代傳統高溫殺菌延長蔬果保存期限，提高食品之安全性還可增加蔬菜原料中單穀胺酸葉酸的含量，使蔬菜中葉酸的結構方便人體吸收，並開發富含單穀胺酸葉酸的蔬菜原料或加工產品。.

(平裝)Subjects--Topical Terms:

1449206
mono/polyglutamate.

高壓加工處理對羽衣甘藍單穀胺酸葉酸含量及生物利用率的影響 = = Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale /
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245 1 0 $a 高壓加工處理對羽衣甘藍單穀胺酸葉酸含量及生物利用率的影響 = $b Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale / $c 黃柏慈.
246 1 1 $a Effect of high-pressure processing on monoglutamate folate content and bioavailability of kale.
250 $a 初版.
260 # $a 雲林縣 : $b 國立虎尾科技大學 , $c 民113.07.
300 $a [9], 104面 : $b 圖, 表 ; $c 30公分.
500 $a 指導教授: 王鐘毅.
500 $a 學年度: 112.
502 $a 碩士論文--國立虎尾科技大學生物科技系碩士班.
504 $a 含參考書目.
520 3 $a 葉酸 ( Folate ) 是一種水溶性維生素，俗稱維生素 B9，是參與人體內許多重要生物反應的必須微量營養素。但人體無法自行合成葉酸，因此需透過每日的飲食或是營養保健品來補充，而綠色蔬菜是攝取天然葉酸的重要來源。一般成年人建議每日攝取 400 微克的葉酸，若長期攝取不足可能會增加罹患心血管疾病、癌症及神經管缺陷的風險。本研究利用高壓加工技術 ( High pressure processing, HPP ) 提升羽衣甘藍 ( Brassica oleracea var. sabellica ) 中天然葉酸的含量，並透過微生物法及高效液相層析法分析蔬菜中單穀胺酸及聚穀胺酸的含量。研究結果顯示，高壓導致蔬菜中的長鏈聚穀胺酸轉變為短鏈及單穀胺酸形式的葉酸。在不同壓力相同時間處理下 200 及 400 MPa 總葉酸含量為 237.06 及 236.32 μg/100 g，而 600 MPa 高壓處理 5 分鐘及 10 分鐘顯著提高羽衣甘藍中總葉酸的含量，分別由控制組的 219.64 μg/100 g 增加至 239.41 及 241.14 μg/100 g， 15 分鐘及 20 分鐘高壓處理總葉酸含量的變化較不顯著。此外，經熱處理後羽衣甘藍聚穀胺酸含量顯著提高、單穀胺酸含量顯著降低，而蔬菜冷凍 24 小時後雖然因解凍過程的水分流失導致總葉酸含量下降，卻也因冷凍使細胞損傷促使內源性酵素作用進而促進聚穀胺酸轉化為單穀胺酸。結果表示，葉酸具熱敏感性，可能會隨著溫度升高而產生降解。而高壓加工技術作為一種新興的非熱加工技術不僅可取代傳統高溫殺菌延長蔬果保存期限，提高食品之安全性還可增加蔬菜原料中單穀胺酸葉酸的含量，使蔬菜中葉酸的結構方便人體吸收，並開發富含單穀胺酸葉酸的蔬菜原料或加工產品。.
520 3 $a Folic acid ( Folate ) is a water-soluble vitamin, commonly known as vitamin B9. It is an essential micronutrient involved in many important biological reactions in the human body. However, the human body cannot synthesize folic acid on its own, so it needs to be supplemented through daily diet or nutritional supplements. Green vegetables are an important source of natural folic acid. The recommended daily intake for adults is 400 micrograms. Long-term insufficient intake may increase the risk of cardiovascular disease, cancer, and neural tube defects. This study used high-pressure processing ( HPP ) technology to increase kale’s natural folic acid content ( Brassica oleracea var. sabellica ). The content of mono-glutamate and poly-glutamate forms of folate in the vegetable was analyzed using microbiological assays and high-performance liquid chromatography ( HPLC ). The results showed that high pressure caused the conversion of long-chain poly-glutamates into short-chain and mono-glutamate forms of folate. Under treatment with the same duration at different pressures, the total folate content at 200 MPa and 400 MPa was 237.06 and 236.32 μg/100 g, respectively. A significant increase in total folate content in kale was observed under 600 MPa high-pressure treatment for 5 minutes and 10 minutes, with levels increasing from 219.64 μg/100 g in the control group to 239.41 and 241.14 μg/100 g, respectively. The changes in total folate content were less significant with 15 and 20 minutes of high-pressure treatment. Additionally, after heat treatment, the poly-glutamate content in kale significantly increased, while the mono-glutamate content significantly decreased. Although freezing the vegetable for 24 hours led to a decrease in total folate content due to moisture loss during the thawing process, it also caused cell damage that promoted the enzymatic conversion of poly-glutamates to mono-glutamates. The results indicate that folate is heat-sensitive and may degrade with rising temperatures. As a novel non-thermal processing technology, HPP not only has the potential to replace traditional high-temperature sterilization to extend the shelf life of fruits and vegetables and improve food safety but also to increase the content of mono-glutamate folate in vegetable raw materials. This makes the folate in vegetables more bioavailable and allows the development of vegetable-based raw materials or processed products rich in monoglutamate folate..
563 $a (平裝)
650 # 4 $a mono/polyglutamate. $3 1449206
650 # 4 $a 5-methyltetrahydrofolate. $3 1449205
650 # 4 $a High pressure processing. $3 1381343
650 # 4 $a Folate. $3 1449204
650 # 4 $a Kale. $3 1449203
650 # 4 $a 單/聚穀胺酸. $3 1449202
650 # 4 $a 高壓加工. $3 1381337
650 # 4 $a 葉酸. $3 1449201
650 # 4 $a 羽衣甘藍. $3 1449200
650 # 4 $a 5-甲基四氫葉酸. $3 1449199
856 7 # $u https://handle.ncl.edu.tw/11296/43g78z $z 電子資源 $2 http