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Microbiological Production of Polyhydroxylbutyrate and Pectinase From Agricultural Wastes.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Microbiological Production of Polyhydroxylbutyrate and Pectinase From Agricultural Wastes./
Author:	Agboola, Florence Olukemi.
Description:	1 online resource (166 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-05, Section: B.
Contained By:	Dissertations Abstracts International85-05B.
Subject:	Microbiology. -
Online resource:	click for full text (PQDT)
ISBN:	9798380848312

Microbiological Production of Polyhydroxylbutyrate and Pectinase From Agricultural Wastes.
Agboola, Florence Olukemi.

Microbiological Production of Polyhydroxylbutyrate and Pectinase From Agricultural Wastes. - 1 online resource (166 pages)

Source: Dissertations Abstracts International, Volume: 85-05, Section: B.

Thesis (Ph.D.)--Kwara State University (Nigeria), 2023.

Includes bibliographical references

Indiscriminate disposal of synthetic polymers, such as synthetic plastic, and an increase in agricultural activities with leftover wastes constitute a major source of environmental pollution. Harnessing agricultural waste easily degradable to produce biopolymer and pectinase can reduce the negative effect of these pollutants. This study aimed at production of biopolymer and pectinase from agricultural wastes (outer peels) by microbiological methods. Microbial isolates were obtained from dump sites soil (surface soil), characterized and identified. The bacterial isolates were screened for biopolymer and pectinase production using standard methods. The pH and temperature were optimized to study their effect on pectinase activities using standard procedures and pectinase was purified using ammonium sulphate and column sephadex G-100. Molecular identification was carried out on the bacterial and fungal isolates using 16S rRNA and 18S rRNA sequences respectively. The effects of varying pH and temperatures on the activity of pectinase were determined. Polymer analysis was carried out using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning electron microscope (SEM). The study revealed that total bacterial counts was 20.50 ± 4.95x108 cfu/mL in dump site 1 (Ipata Market), while the total fungal counts was 36.50±2.12x105 sfu/mL from sample site 2 (Unity road). The bacterial isolatesidentified were Bacillus subtilis strain AGB1, Bacillus megaterium strain AGB2, Paenibacillus polymyxa strain AGB3, Bacillus licheniformis strain AGB4, Bacillus cereus strain AGB5, Bacillus mycoides strain AGB6, and Enterobacter species. The fungal isolates were Clasdoporium tenuissimum AGF1, Penicillium chrysogenum strain AGF2, Aspergillus niger strain AGF3, Rhizopus specie strain AGF4, and Penicillium marneffie strain AGF5. Bacterial isolates (Bacillus subtilis strain AGB1(OM273871), Bacillus megaterium strain AGB2 (OM216844), Paenibacillus polymyxa strain AGB3 (OM273889) and Bacillus licheniformis strain AGB4 (OP703543)}, were able to produce biopolymer and pectinase, while three fungal isolates, Clasdoporium tenuissimum strain AGF1, Penicillium chrysogenum strain AGF2 (OM273982), and Aspergillus niger strain AGF3 (OM273994), produced pectinase. The optimum temperature for pectinase production were recorded at 30oC and 40oC for bacteria at pH 5 and 7, 40oC, and 50oC for fungi respectively. Aspergillus niger strain AGF3 (OM273994) had the highest specific pectinase activity (2300 U/mg) using mango as source of waste, while the least (1000 U/mg) specific activity was produced by Penicillium chrysogenum strain AGF2 (OM273982) from plantain outer peel. The highest specific activity (1000 U/mg) was produced by all the bacteria species from all the wastes (mango, orange, banana, lemon, plantain, watermelon, shaddock, pineapple and sweet potato outer peel) at 30oC, 40oC and at pH 7. Fourier Transform Infrared Spectroscopy (FTIR) indicated that the biopolymer, polyhydroxylbutyrate (PHB) belonged to carbonyl functional group (C=O), while SEM showed that it had crystalline surfaces. The study concluded that Bacillus licheniformis strain (OP703543) and Bacillus megaterium strain AGB2 (OM216844) isolates could serve as alternative sources of polyhydroxylbutyrate production, while the fungal isolates could be explored for pectinase production, when cultured on selected agricultural wastes under optimized fermentation conditions.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798380848312Subjects--Topical Terms:

591510
Microbiology.
Subjects--Index Terms:

Microbiological productionIndex Terms--Genre/Form:

554714
Electronic books.

Microbiological Production of Polyhydroxylbutyrate and Pectinase From Agricultural Wastes.
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504 $a Includes bibliographical references
520 $a Indiscriminate disposal of synthetic polymers, such as synthetic plastic, and an increase in agricultural activities with leftover wastes constitute a major source of environmental pollution. Harnessing agricultural waste easily degradable to produce biopolymer and pectinase can reduce the negative effect of these pollutants. This study aimed at production of biopolymer and pectinase from agricultural wastes (outer peels) by microbiological methods. Microbial isolates were obtained from dump sites soil (surface soil), characterized and identified. The bacterial isolates were screened for biopolymer and pectinase production using standard methods. The pH and temperature were optimized to study their effect on pectinase activities using standard procedures and pectinase was purified using ammonium sulphate and column sephadex G-100. Molecular identification was carried out on the bacterial and fungal isolates using 16S rRNA and 18S rRNA sequences respectively. The effects of varying pH and temperatures on the activity of pectinase were determined. Polymer analysis was carried out using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning electron microscope (SEM). The study revealed that total bacterial counts was 20.50 ± 4.95x108 cfu/mL in dump site 1 (Ipata Market), while the total fungal counts was 36.50±2.12x105 sfu/mL from sample site 2 (Unity road). The bacterial isolatesidentified were Bacillus subtilis strain AGB1, Bacillus megaterium strain AGB2, Paenibacillus polymyxa strain AGB3, Bacillus licheniformis strain AGB4, Bacillus cereus strain AGB5, Bacillus mycoides strain AGB6, and Enterobacter species. The fungal isolates were Clasdoporium tenuissimum AGF1, Penicillium chrysogenum strain AGF2, Aspergillus niger strain AGF3, Rhizopus specie strain AGF4, and Penicillium marneffie strain AGF5. Bacterial isolates (Bacillus subtilis strain AGB1(OM273871), Bacillus megaterium strain AGB2 (OM216844), Paenibacillus polymyxa strain AGB3 (OM273889) and Bacillus licheniformis strain AGB4 (OP703543)}, were able to produce biopolymer and pectinase, while three fungal isolates, Clasdoporium tenuissimum strain AGF1, Penicillium chrysogenum strain AGF2 (OM273982), and Aspergillus niger strain AGF3 (OM273994), produced pectinase. The optimum temperature for pectinase production were recorded at 30oC and 40oC for bacteria at pH 5 and 7, 40oC, and 50oC for fungi respectively. Aspergillus niger strain AGF3 (OM273994) had the highest specific pectinase activity (2300 U/mg) using mango as source of waste, while the least (1000 U/mg) specific activity was produced by Penicillium chrysogenum strain AGF2 (OM273982) from plantain outer peel. The highest specific activity (1000 U/mg) was produced by all the bacteria species from all the wastes (mango, orange, banana, lemon, plantain, watermelon, shaddock, pineapple and sweet potato outer peel) at 30oC, 40oC and at pH 7. Fourier Transform Infrared Spectroscopy (FTIR) indicated that the biopolymer, polyhydroxylbutyrate (PHB) belonged to carbonyl functional group (C=O), while SEM showed that it had crystalline surfaces. The study concluded that Bacillus licheniformis strain (OP703543) and Bacillus megaterium strain AGB2 (OM216844) isolates could serve as alternative sources of polyhydroxylbutyrate production, while the fungal isolates could be explored for pectinase production, when cultured on selected agricultural wastes under optimized fermentation conditions.
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653 $a Polyhydroxylbutyrate
653 $a Biopolymer
653 $a Fourier Transform Infrared Spectroscopy
653 $a Leftover wastes
653 $a Agricultural wastes
653 $a Pectinase
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