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From In Situ to In Vitro : = Measuring Contact-Dependent Determinants of Human Natural Killer Cell Development.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	From In Situ to In Vitro :/
Reminder of title:	Measuring Contact-Dependent Determinants of Human Natural Killer Cell Development.
Author:	Hegewisch Solloa, Everardo.
Description:	1 online resource (226 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
Contained By:	Dissertations Abstracts International85-06B.
Subject:	Immunology. -
Online resource:	click for full text (PQDT)
ISBN:	9798381171969

From In Situ to In Vitro : = Measuring Contact-Dependent Determinants of Human Natural Killer Cell Development.
Hegewisch Solloa, Everardo.

From In Situ to In Vitro :Measuring Contact-Dependent Determinants of Human Natural Killer Cell Development. - 1 online resource (226 pages)

Source: Dissertations Abstracts International, Volume: 85-06, Section: B.

Thesis (Ph.D.)--Columbia University, 2024.

Includes bibliographical references

Human natural killer (NK) cells are found in virtually all tissues where they act as a first line defense against malignant and virally infected cells. The development of NK cells from CD34+ hematopoietic progenitors is a complex process that involves navigating through different microenvironments and requires contact-dependent interactions with stromal cells. The molecular mediators of NK cell developmental subset trafficking, cell-cell interactions, and maturation have not been fully characterized. This thesis presents 3 studies that aim to uncover contact-dependent interactions that drive human NK cell development. Chapter 2 focuses on defining the adhesome profile of human NK cells from in vitro derived populations, tonsil, and peripheral blood. This study reveals that the tissue origin and developmental stage of NK cells influence the expression of adhesome-associated genes and proteins, as well as the content of cortical actin, which suggests a link between adhesome expression and actin regulation in NK cells. Chapter 3 presents the first comprehensive study on human NK cell development in pediatric tonsil using cyclic immunofluorescence microscopy and imaging mass cytometry. We reveal that NK cell subset localization and interactions are dependent on NK cell developmental stage and tissue residency. Chapter 4 demonstrates that neural cell adhesion molecule (NCAM) on stromal cells promotes maintenance of a mesenchymal-like state and subsequently the survival and proliferation of human NK cell precursors. Overall, this thesis provides new insights on previously unknown mediators of NK cell contact-dependent interactions and unveils the first road map of in situ NK cell development.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798381171969Subjects--Topical Terms:

592892
Immunology.
Subjects--Index Terms:

Innate immunityIndex Terms--Genre/Form:

554714
Electronic books.

From In Situ to In Vitro : = Measuring Contact-Dependent Determinants of Human Natural Killer Cell Development.
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