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Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells./
Author:	Liang, Zhiyu.
Description:	1 online resource (115 pages)
Notes:	Source: Masters Abstracts International, Volume: 84-10.
Contained By:	Masters Abstracts International84-10.
Subject:	Tumor necrosis factor-TNF. -
Online resource:	click for full text (PQDT)
ISBN:	9798377679912

Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells.
Liang, Zhiyu.

Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells. - 1 online resource (115 pages)

Source: Masters Abstracts International, Volume: 84-10.

Thesis (M.E.)--The University of Western Ontario (Canada), 2022.

Includes bibliographical references

The cell microenvironment plays a critical role in modulating adipose-derived stromal cell (ASC) proliferation and paracrine function. The current study investigated the effects of decellularized adipose tissue (DAT) coatings, low-level oscillatory shear stress (~0.04‑0.3 dyn/cm2), hypoxia (2% O2), and pro-inflammatory cytokine priming with IFN-g and TNF-a on human ASC proliferation and paracrine factor secretion in the context of a rocking bioreactor. Culturing under 20% O2 resulted in a higher cell density after 7 days of culture. Without cytokine priming, the varying culture conditions significantly impacted the levels of the pro-angiogenic factors VEGF, HGF, and angiogenin detected in conditioned media samples. In contrast, when the cells were primed, the levels of the immunomodulatory factors IL-6 and IL-8 were most affected by the varying microenvironmental factors. Overall, a novel bioreactor system was developed for ASC expansion and preconditioning, demonstrating that the cell microenvironment could be tuned to modulate ASC paracrine factor secretion.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798377679912Subjects--Topical Terms:

1465884
Tumor necrosis factor-TNF.
Index Terms--Genre/Form:

554714
Electronic books.

Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells.
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245 1 0 $a Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells.
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500 $a Source: Masters Abstracts International, Volume: 84-10.
500 $a Advisor: Flynn, Lauren.
502 $a Thesis (M.E.)--The University of Western Ontario (Canada), 2022.
504 $a Includes bibliographical references
520 $a The cell microenvironment plays a critical role in modulating adipose-derived stromal cell (ASC) proliferation and paracrine function. The current study investigated the effects of decellularized adipose tissue (DAT) coatings, low-level oscillatory shear stress (~0.04‑0.3 dyn/cm2), hypoxia (2% O2), and pro-inflammatory cytokine priming with IFN-g and TNF-a on human ASC proliferation and paracrine factor secretion in the context of a rocking bioreactor. Culturing under 20% O2 resulted in a higher cell density after 7 days of culture. Without cytokine priming, the varying culture conditions significantly impacted the levels of the pro-angiogenic factors VEGF, HGF, and angiogenin detected in conditioned media samples. In contrast, when the cells were primed, the levels of the immunomodulatory factors IL-6 and IL-8 were most affected by the varying microenvironmental factors. Overall, a novel bioreactor system was developed for ASC expansion and preconditioning, demonstrating that the cell microenvironment could be tuned to modulate ASC paracrine factor secretion.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Tumor necrosis factor-TNF. $3 1465884
650 4 $a Vascular endothelial growth factor. $3 1475199
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650 4 $a Cell growth. $3 1475200
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710 2 $a The University of Western Ontario (Canada). $3 1184598
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