國立虎尾科技大學 |

Microbial and Metabolic Dynamics in Sponges Under Ocean Acidification.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Microbial and Metabolic Dynamics in Sponges Under Ocean Acidification./
Author:	Efremova, Jana.
Description:	1 online resource (235 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
Contained By:	Dissertations Abstracts International85-06B.
Subject:	Microbiology. -
Online resource:	click for full text (PQDT)
ISBN:	9798380869485

Microbial and Metabolic Dynamics in Sponges Under Ocean Acidification.
Efremova, Jana.

Microbial and Metabolic Dynamics in Sponges Under Ocean Acidification. - 1 online resource (235 pages)

Source: Dissertations Abstracts International, Volume: 85-06, Section: B.

Thesis (Ph.D.)--Open University (United Kingdom), 2023.

Includes bibliographical references

Ocean acidification (OA) poses a significant threat to marine ecosystems, and benthic organisms must develop adaptive strategies. Despite being frequently regarded as 'winner taxa', we lack a comprehensive understanding of how sponges tolerate stress and undergo positive acclimatization. Marine sponges can be categorized as high or low microbial abundance (HMA or LMA) species, which may adopt distinct strategies to maintain homeostasis and fitness under changing conditions. This study investigated adaptive traits of the HMA sponge Chondrosia reniformis and the LMA sponge Spirastrella cunctatrix by comparing microbial and metabolic shifts in sponge holobionts collected from a natural CO2 vent system and a control pH site in Ischia, Italy. Microbial diversity and core microbiomes changed for both species in response to OA. Morphologically, S. cunctatrix exhibited tissue necrosis accompanied by reduced oscula and water canal sizes, indicating a stress-induced dysbiosis and microbial instability. In contrast, C. reniformis appeared to benefit from a highly diverse microbiome with functional redundancy and local microbiome stability, promoting acclimatization to OA. NMR-based metabolomics revealed stable metabolite profiles across sites for C. reniformis, indicating metabolic homeostasis, whereas metabolic shifts in S. cunctatrix suggested OA interference in several pathways, including osmoregulation and energy metabolism. To expand on organismal acclimatization processes towards biochemical exchanges with the environment, a new in situ sampling methodology was developed. The study of inhaled and exhaled water fluxes of filter-feeders was improved using a submarine peristaltic pump, which was tested on C. reniformis. The new methodology can be applied to various ecological research topics, such as nutrient cycling, filter-feeding fluxes, plankton dynamics, and seawater metabolomics. This dissertation compares diverse OA acclimatization strategies of two co-occurring Porifera species in a CO2 vent system based on microbiome and metabolic patterns. Moreover, parallel studies of biochemical exchanges with seawater are crucial to reconstruct these adaptation mechanisms.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798380869485Subjects--Topical Terms:

591510
Microbiology.
Subjects--Index Terms:

Ocean acidificationIndex Terms--Genre/Form:

554714
Electronic books.

Microbial and Metabolic Dynamics in Sponges Under Ocean Acidification.
LDR:03592ntm a22003977 4500 001 1150316
005 20241028114745.5
006 m o d
007 cr bn ---uuuuu
008 250605s2023 xx obm 000 0 eng d
020 $a 9798380869485
035 $a (MiAaPQ)AAI30943009
035 $a (MiAaPQ)Open_Univ_UK92001
035 $a AAI30943009
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Efremova, Jana. $3 1476778
245 1 0 $a Microbial and Metabolic Dynamics in Sponges Under Ocean Acidification.
264 0 $c 2023
300 $a 1 online resource (235 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 85-06, Section: B.
500 $a Advisor: Cardini, Ulisse.
502 $a Thesis (Ph.D.)--Open University (United Kingdom), 2023.
504 $a Includes bibliographical references
520 $a Ocean acidification (OA) poses a significant threat to marine ecosystems, and benthic organisms must develop adaptive strategies. Despite being frequently regarded as 'winner taxa', we lack a comprehensive understanding of how sponges tolerate stress and undergo positive acclimatization. Marine sponges can be categorized as high or low microbial abundance (HMA or LMA) species, which may adopt distinct strategies to maintain homeostasis and fitness under changing conditions. This study investigated adaptive traits of the HMA sponge Chondrosia reniformis and the LMA sponge Spirastrella cunctatrix by comparing microbial and metabolic shifts in sponge holobionts collected from a natural CO2 vent system and a control pH site in Ischia, Italy. Microbial diversity and core microbiomes changed for both species in response to OA. Morphologically, S. cunctatrix exhibited tissue necrosis accompanied by reduced oscula and water canal sizes, indicating a stress-induced dysbiosis and microbial instability. In contrast, C. reniformis appeared to benefit from a highly diverse microbiome with functional redundancy and local microbiome stability, promoting acclimatization to OA. NMR-based metabolomics revealed stable metabolite profiles across sites for C. reniformis, indicating metabolic homeostasis, whereas metabolic shifts in S. cunctatrix suggested OA interference in several pathways, including osmoregulation and energy metabolism. To expand on organismal acclimatization processes towards biochemical exchanges with the environment, a new in situ sampling methodology was developed. The study of inhaled and exhaled water fluxes of filter-feeders was improved using a submarine peristaltic pump, which was tested on C. reniformis. The new methodology can be applied to various ecological research topics, such as nutrient cycling, filter-feeding fluxes, plankton dynamics, and seawater metabolomics. This dissertation compares diverse OA acclimatization strategies of two co-occurring Porifera species in a CO2 vent system based on microbiome and metabolic patterns. Moreover, parallel studies of biochemical exchanges with seawater are crucial to reconstruct these adaptation mechanisms.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Microbiology. $3 591510
650 4 $a Ecology. $3 575279
650 4 $a Marine geology. $3 1178828
653 $a Ocean acidification
653 $a Microbial abundance
653 $a Microbiome
653 $a Filter-feeding fluxes
655 7 $a Electronic books. $2 local $3 554714
690 $a 0410
690 $a 0556
690 $a 0329
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Open University (United Kingdom). $3 1335296
773 0 $t Dissertations Abstracts International $g 85-06B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30943009 $z click for full text (PQDT)