國立虎尾科技大學 |

Design and optimization of PEGDA as a functional material for 3D printing of liver tissue.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Design and optimization of PEGDA as a functional material for 3D printing of liver tissue./
Author:	Anandakrishnan, Nanditha.
Description:	1 online resource (62 pages)
Notes:	Source: Masters Abstracts International, Volume: 77-05.
Contained By:	Masters Abstracts International77-05.
Subject:	Biomedical engineering. -
Online resource:	click for full text (PQDT)
ISBN:	9781339103044

Design and optimization of PEGDA as a functional material for 3D printing of liver tissue.
Anandakrishnan, Nanditha.

Design and optimization of PEGDA as a functional material for 3D printing of liver tissue. - 1 online resource (62 pages)

Source: Masters Abstracts International, Volume: 77-05.

Thesis (M.S.)--State University of New York at Buffalo, 2015.

Includes bibliographical references

Clinical drug development suffers largely due to the inability of in vitro 2D culture studies to precisely predict the physiological effect of drug toxicity. Three dimensional (3D) cell culture can provide solution to this problem since behaviour of cells in 3D culture closely resemble their behaviour in physiological conditions. This study investigates the application of a synthetic extracellular matrix (ECM) mimic as a three dimensional environment for culturing liver cells expressing considerable functionality. The synthetic hydrogel, polyethylene glycol diacrylate (PEGDA) was used in this study as a support environment for the cells. PEGDA of two different molecular weights (4000 Da and 400 Da) and their composites at different weight percentage ratio were investigated for their swelling properties, pore size and cell viability. Since liver architecture is paramount for its effective functioning, projection based stereolithographic printing is used in this study for printing complex 3D structures. Based on the results, 30 wt % PEGDA MW 4000 Da was chosen to assess liver functionality as they were biocompatible and also showed promise to be optimized for printing complex structures. Liver cell functionality was assessed using Enzyme linked immunosorbent assay (ELISA) for albumin secretion and Cytochrome P450 enzyme assay. While studying heterotypic cell-cell interactions by co-culturing fibroblasts with liver cells, it was observed that fibroblasts indeed enhanced the functionality of the liver cells encapsulated in the hydrogel. Based on the results of this investigation, it can be inferred that PEGDA can serve as a 3D printable, bio-functional liver cell scaffold.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9781339103044Subjects--Topical Terms:

588770
Biomedical engineering.
Subjects--Index Terms:

3D cell cultureIndex Terms--Genre/Form:

554714
Electronic books.

Design and optimization of PEGDA as a functional material for 3D printing of liver tissue.
LDR:03136ntm a22004097 4500 001 1150977
005 20241104055640.5
006 m o d
007 cr bn ---uuuuu
008 250605s2015 xx obm 000 0 eng d
020 $a 9781339103044
035 $a (MiAaPQ)AAI1600730
035 $a (MiAaPQ)buffalo:14141
035 $a AAI1600730
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Anandakrishnan, Nanditha. $3 1477625
245 1 0 $a Design and optimization of PEGDA as a functional material for 3D printing of liver tissue.
264 0 $c 2015
300 $a 1 online resource (62 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 77-05.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Zhao, Ruogang.
502 $a Thesis (M.S.)--State University of New York at Buffalo, 2015.
504 $a Includes bibliographical references
520 $a Clinical drug development suffers largely due to the inability of in vitro 2D culture studies to precisely predict the physiological effect of drug toxicity. Three dimensional (3D) cell culture can provide solution to this problem since behaviour of cells in 3D culture closely resemble their behaviour in physiological conditions. This study investigates the application of a synthetic extracellular matrix (ECM) mimic as a three dimensional environment for culturing liver cells expressing considerable functionality. The synthetic hydrogel, polyethylene glycol diacrylate (PEGDA) was used in this study as a support environment for the cells. PEGDA of two different molecular weights (4000 Da and 400 Da) and their composites at different weight percentage ratio were investigated for their swelling properties, pore size and cell viability. Since liver architecture is paramount for its effective functioning, projection based stereolithographic printing is used in this study for printing complex 3D structures. Based on the results, 30 wt % PEGDA MW 4000 Da was chosen to assess liver functionality as they were biocompatible and also showed promise to be optimized for printing complex structures. Liver cell functionality was assessed using Enzyme linked immunosorbent assay (ELISA) for albumin secretion and Cytochrome P450 enzyme assay. While studying heterotypic cell-cell interactions by co-culturing fibroblasts with liver cells, it was observed that fibroblasts indeed enhanced the functionality of the liver cells encapsulated in the hydrogel. Based on the results of this investigation, it can be inferred that PEGDA can serve as a 3D printable, bio-functional liver cell scaffold.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Biomedical engineering. $3 588770
653 $a 3D cell culture
653 $a 3D printing
653 $a Hydrogels
653 $a Liver
653 $a Polyethylene glycol diacrylate
653 $a Scaffold
655 7 $a Electronic books. $2 local $3 554714
690 $a 0541
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a State University of New York at Buffalo. $b Biomedical Engineering. $3 1472694
773 0 $t Masters Abstracts International $g 77-05.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1600730 $z click for full text (PQDT)