國立虎尾科技大學 |

Cell Membrane Engineering With Structural DNA Nanotechnology.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Cell Membrane Engineering With Structural DNA Nanotechnology./
Author:	Wang, Weitao.
Description:	1 online resource (159 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
Contained By:	Dissertations Abstracts International85-04B.
Subject:	Mechanical engineering. -
Online resource:	click for full text (PQDT)
ISBN:	9798380594912

Cell Membrane Engineering With Structural DNA Nanotechnology.
Wang, Weitao.

Cell Membrane Engineering With Structural DNA Nanotechnology. - 1 online resource (159 pages)

Source: Dissertations Abstracts International, Volume: 85-04, Section: B.

Thesis (Ph.D.)--Carnegie Mellon University, 2023.

Includes bibliographical references

Recent advances in structural DNA nanotechnology have opened up new avenues for manipulating cellular systems at nanoscale level. The cell plasma membrane, a critical interface governing cellular communication and material transport between cells and their surroundings, presents a promising target for such interventions. Probing, investigating and thus engineering cell membranes will greatly add our understanding on the important role of membranes to cells and unlock exciting prospects for cell-based therapies.The thesis begins by providing an overview of the fundamental structure and function of the cell membrane, highlighting its importance in cellular physiology and function. It then delves into the principles and techniques of structural DNA nanotechnology, covering synthesis, functional modification, purification and characterization, showcasing its unique ability to design and construct user-defined nanostructures with high programmability and specificity. To apply DNA nanostructures to cell membranes, it is critical to understand the way they interact with each other. Building upon the fundamental concepts, I then present approaches developed both within our groups and in the literature to overcome the physical and chemical barriers of cell membranes, and deliver DNA nanostructures to targeted positions on cells. To gain an in-depth insight into the interactions between DNA nanostructures and cell membranes, we apply the state-of-art microscopy technologies, including scanning conductance microscopy and confocal laser scanning microscopy, to provide the first direct observation of how cells dynamically respond to DNA nanostructures at nano- and micro- scale. We observe significant membrane deformation as a result of the binding of DNA nanostructures onto cell membranes, while remarkably, the overall cell viability remains largely unaffected throughout this process. This finding reinforces the potential of DNA nanostructures as promising candidates for cell membrane engineering and further applications. One potential category of the biomedical applications is to use DNA nanostructures as delivery vehicles for therapeutic agents. To enhance intracellular delivery, a cell-surface binding approach is employed. Compared to bare DNA nanostructures, cell-surface targeted structures demonstrate up to an 8-fold increase in intracellular delivery within just half an hour. Notably, the intranuclear delivery of these structures also experiences a remarkable up to 3-fold improvement. Such an enhancement is consistent across a diverse array of DNA nanostructures, such as DNA nanospheres, nanorods, and nanotiles, and utilizes various membrane attachment methods, including cholesterol membrane anchoring and click glyco- calyx anchoring. These findings pose DNA nanostructures as promising delivery systems for therapeutic agents, proteins, and even genetic material. Lastly, to showcase the potential of DNA nanostructures as a biomaterial for biomedical applications, we construct a synthetic cellular armor utilizing DNA nanorods to enhance the cellular protection against external threats. By recruiting and crosslinking DNA rods on the cell membrane, a versatile and programmable nanoshell is constructed, serving as a robust protective barrier that enhances cell viability when faced with environmental assaults. Interestingly, the nanoshell also exerts influence over the biophysical properties of the membrane, presenting itself as a platform for studying the relationship between membrane biomechanics and cell function.In conclusion, this thesis represents initial steps towards engineering cell membranes using structural DNA nanotechnology, from exploring the cellular response to DNA nanostructures, to leveraging membrane binding for rapid enhancement in the intracellular and intranuclear delivery, to using DNA as a protective biomaterial for cell encapsulation. By harnessing the potential of DNA-based nanoplatforms, we seek to understand how to better interact with cellular membranes, and contribute to new frontiers in membrane manipulation and pave the way for potential transformative advancements in biomedical science.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798380594912Subjects--Topical Terms:

557493
Mechanical engineering.
Subjects--Index Terms:

Cell membranesIndex Terms--Genre/Form:

554714
Electronic books.

Cell Membrane Engineering With Structural DNA Nanotechnology.
LDR:05494ntm a22003737 4500 001 1151845
005 20241118135840.5
006 m o d
007 cr mn ---uuuuu
008 250605s2023 xx obm 000 0 eng d
020 $a 9798380594912
035 $a (MiAaPQ)AAI30574533
035 $a AAI30574533
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Wang, Weitao. $3 1478681
245 1 0 $a Cell Membrane Engineering With Structural DNA Nanotechnology.
264 0 $c 2023
300 $a 1 online resource (159 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
500 $a Advisor: Taylor, Rebecca E.;Ren, Xi.
502 $a Thesis (Ph.D.)--Carnegie Mellon University, 2023.
504 $a Includes bibliographical references
520 $a Recent advances in structural DNA nanotechnology have opened up new avenues for manipulating cellular systems at nanoscale level. The cell plasma membrane, a critical interface governing cellular communication and material transport between cells and their surroundings, presents a promising target for such interventions. Probing, investigating and thus engineering cell membranes will greatly add our understanding on the important role of membranes to cells and unlock exciting prospects for cell-based therapies.The thesis begins by providing an overview of the fundamental structure and function of the cell membrane, highlighting its importance in cellular physiology and function. It then delves into the principles and techniques of structural DNA nanotechnology, covering synthesis, functional modification, purification and characterization, showcasing its unique ability to design and construct user-defined nanostructures with high programmability and specificity. To apply DNA nanostructures to cell membranes, it is critical to understand the way they interact with each other. Building upon the fundamental concepts, I then present approaches developed both within our groups and in the literature to overcome the physical and chemical barriers of cell membranes, and deliver DNA nanostructures to targeted positions on cells. To gain an in-depth insight into the interactions between DNA nanostructures and cell membranes, we apply the state-of-art microscopy technologies, including scanning conductance microscopy and confocal laser scanning microscopy, to provide the first direct observation of how cells dynamically respond to DNA nanostructures at nano- and micro- scale. We observe significant membrane deformation as a result of the binding of DNA nanostructures onto cell membranes, while remarkably, the overall cell viability remains largely unaffected throughout this process. This finding reinforces the potential of DNA nanostructures as promising candidates for cell membrane engineering and further applications. One potential category of the biomedical applications is to use DNA nanostructures as delivery vehicles for therapeutic agents. To enhance intracellular delivery, a cell-surface binding approach is employed. Compared to bare DNA nanostructures, cell-surface targeted structures demonstrate up to an 8-fold increase in intracellular delivery within just half an hour. Notably, the intranuclear delivery of these structures also experiences a remarkable up to 3-fold improvement. Such an enhancement is consistent across a diverse array of DNA nanostructures, such as DNA nanospheres, nanorods, and nanotiles, and utilizes various membrane attachment methods, including cholesterol membrane anchoring and click glyco- calyx anchoring. These findings pose DNA nanostructures as promising delivery systems for therapeutic agents, proteins, and even genetic material. Lastly, to showcase the potential of DNA nanostructures as a biomaterial for biomedical applications, we construct a synthetic cellular armor utilizing DNA nanorods to enhance the cellular protection against external threats. By recruiting and crosslinking DNA rods on the cell membrane, a versatile and programmable nanoshell is constructed, serving as a robust protective barrier that enhances cell viability when faced with environmental assaults. Interestingly, the nanoshell also exerts influence over the biophysical properties of the membrane, presenting itself as a platform for studying the relationship between membrane biomechanics and cell function.In conclusion, this thesis represents initial steps towards engineering cell membranes using structural DNA nanotechnology, from exploring the cellular response to DNA nanostructures, to leveraging membrane binding for rapid enhancement in the intracellular and intranuclear delivery, to using DNA as a protective biomaterial for cell encapsulation. By harnessing the potential of DNA-based nanoplatforms, we seek to understand how to better interact with cellular membranes, and contribute to new frontiers in membrane manipulation and pave the way for potential transformative advancements in biomedical science.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Mechanical engineering. $3 557493
650 4 $a Biomedical engineering. $3 588770
650 4 $a Nanotechnology. $3 557660
653 $a Cell membranes
653 $a DNA nanostructures
653 $a Cell encapsulation
655 7 $a Electronic books. $2 local $3 554714
690 $a 0548
690 $a 0541
690 $a 0652
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Carnegie Mellon University. $b Mechanical Engineering. $3 1148639
773 0 $t Dissertations Abstracts International $g 85-04B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30574533 $z click for full text (PQDT)