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Maintaining Nitrogen-Limited Balanced-Growth States in a Cyclo-Turbidostat.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Maintaining Nitrogen-Limited Balanced-Growth States in a Cyclo-Turbidostat./
Author:	Franks, Dylan.
Description:	1 online resource (104 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-02, Section: B.
Contained By:	Dissertations Abstracts International85-02B.
Subject:	Plant sciences. -
Online resource:	click for full text (PQDT)
ISBN:	9798380080934

Maintaining Nitrogen-Limited Balanced-Growth States in a Cyclo-Turbidostat.
Franks, Dylan.

Maintaining Nitrogen-Limited Balanced-Growth States in a Cyclo-Turbidostat. - 1 online resource (104 pages)

Source: Dissertations Abstracts International, Volume: 85-02, Section: B.

Thesis (Ph.D.)--Oklahoma State University, 2023.

Includes bibliographical references

This dissertation presents two separate studies on the green microalga Picochlorum oklahomense. The first study investigates the minimum nitrogen cell quota required to support maximum growth rate and the effects of nitrogen limitation on cycloturbidostat cultures under steady-state, balanced growth conditions. This study introduces the concept of the threshold of nitrogen cell quota necessary to support maximum growth rate denoted as QN, T. The second study provides empirical evidence of multiple fission in Picochlorum oklahomense grown in light-saturated turbidostats and in semi-continuous cultures. We present evidence that Picochlorum oklahomense is an autosporic species and produces 2 to 4 daughter cells per mother cell via a multiple fission pathway. Additionally, the paper discusses the utility of turbidostats in experimental phycology and provides an outline of cycloturbidostat fundamentals. Lastly, this dissertation uses unpublished data from preliminary trials to describe potential issues encountered when utilizing cycloturbidostats in the laboratory, particularly in nutrientlimitation studies. The study highlights the importance of microalgae in understanding algal biology and building valuable year-round supply chains.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2024

Mode of access: World Wide Web

ISBN: 9798380080934Subjects--Topical Terms:

1179743
Plant sciences.
Subjects--Index Terms:

Balanced growthIndex Terms--Genre/Form:

554714
Electronic books.

Maintaining Nitrogen-Limited Balanced-Growth States in a Cyclo-Turbidostat.
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500 $a Advisor: Henley, William.
502 $a Thesis (Ph.D.)--Oklahoma State University, 2023.
504 $a Includes bibliographical references
520 $a This dissertation presents two separate studies on the green microalga Picochlorum oklahomense. The first study investigates the minimum nitrogen cell quota required to support maximum growth rate and the effects of nitrogen limitation on cycloturbidostat cultures under steady-state, balanced growth conditions. This study introduces the concept of the threshold of nitrogen cell quota necessary to support maximum growth rate denoted as QN, T. The second study provides empirical evidence of multiple fission in Picochlorum oklahomense grown in light-saturated turbidostats and in semi-continuous cultures. We present evidence that Picochlorum oklahomense is an autosporic species and produces 2 to 4 daughter cells per mother cell via a multiple fission pathway. Additionally, the paper discusses the utility of turbidostats in experimental phycology and provides an outline of cycloturbidostat fundamentals. Lastly, this dissertation uses unpublished data from preliminary trials to describe potential issues encountered when utilizing cycloturbidostats in the laboratory, particularly in nutrientlimitation studies. The study highlights the importance of microalgae in understanding algal biology and building valuable year-round supply chains.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2024
538 $a Mode of access: World Wide Web
650 4 $a Plant sciences. $3 1179743
650 4 $a Chemistry. $3 593913
650 4 $a Biochemistry. $3 582831
653 $a Balanced growth
653 $a Continuous culture
653 $a Nitrogen limitation
653 $a Uncoupling
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710 2 $a Oklahoma State University. $b Plant Science (Ph.D). $3 1478860
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