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In situ electrokinetic sample preparation for self-assembled monolayer based electrochemical biosensing.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	In situ electrokinetic sample preparation for self-assembled monolayer based electrochemical biosensing./
Author:	Sin, Lai Yi Mandy.
Description:	144 p.
Notes:	Source: Dissertation Abstracts International, Volume: 72-12, Section: B, page: 7645.
Contained By:	Dissertation Abstracts International72-12B.
Subject:	Engineering, Mechanical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3473641
ISBN:	9781124882116

In situ electrokinetic sample preparation for self-assembled monolayer based electrochemical biosensing.
Sin, Lai Yi Mandy.

In situ electrokinetic sample preparation for self-assembled monolayer based electrochemical biosensing. - 144 p.

Source: Dissertation Abstracts International, Volume: 72-12, Section: B, page: 7645.

Thesis (Ph.D.)--The University of Arizona, 2011.

Electrokinetics based microfluidic systems are potentially promising for lab-on-a-chip applications due to their effectiveness in manipulating nanoscale and biological objects, label-free operation, simple fabrication processes, small voltage requirements, and most importantly simple system integration strategy. Among various electrokinetics techniques, AC electrothermal flow (ACEF) is the most promising technique in microfluidic manipulation toward biomedical applications due to its effectiveness in high conductivity biological and physiological fluids. As relatively little is known about the ACEF induced fluid motion at highly conductive samples, the characteristics of electrothermal manipulation of fluid samples with different conductivities were investigated systematically. For low conductivity sample (below 1 S/m), the characteristics of the electrothermal fluid motion was in quantitative agreement with the theory. For high conductivity samples (greater than 1 S/m), the fluid motion appeared to deviate from the model as a result of electrochemical reactions and the temperature effect. Here, a universal electrode approach which directly implements ACEF-induced sample preparation on a SAM based electrochemical sensor for point-of-care diagnostics of urinary tract infections has also been demonstrated. Using uropathogenic E. coli clinical isolates as model systems, we demonstrate that "on-chip" ACEF-induced sample preparation can improve the sensor performance without complicated system integration strategy and presents a pathway for implementing truly lab on a chip, instead of chip in a lab. Finally an integrated chip approach has been proposed for transforming 26 electrochemical sensing system from laboratory research into point-of-care diagnostics with multiple microelectrodes.

ISBN: 9781124882116Subjects--Topical Terms:

845387
Engineering, Mechanical.

In situ electrokinetic sample preparation for self-assembled monolayer based electrochemical biosensing.
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035 $a (UMI)AAI3473641
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100 1 $a Sin, Lai Yi Mandy. $3 845456
245 1 0 $a In situ electrokinetic sample preparation for self-assembled monolayer based electrochemical biosensing.
300 $a 144 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-12, Section: B, page: 7645.
500 $a Adviser: Pak Kin Wong.
502 $a Thesis (Ph.D.)--The University of Arizona, 2011.
520 $a Electrokinetics based microfluidic systems are potentially promising for lab-on-a-chip applications due to their effectiveness in manipulating nanoscale and biological objects, label-free operation, simple fabrication processes, small voltage requirements, and most importantly simple system integration strategy. Among various electrokinetics techniques, AC electrothermal flow (ACEF) is the most promising technique in microfluidic manipulation toward biomedical applications due to its effectiveness in high conductivity biological and physiological fluids. As relatively little is known about the ACEF induced fluid motion at highly conductive samples, the characteristics of electrothermal manipulation of fluid samples with different conductivities were investigated systematically. For low conductivity sample (below 1 S/m), the characteristics of the electrothermal fluid motion was in quantitative agreement with the theory. For high conductivity samples (greater than 1 S/m), the fluid motion appeared to deviate from the model as a result of electrochemical reactions and the temperature effect. Here, a universal electrode approach which directly implements ACEF-induced sample preparation on a SAM based electrochemical sensor for point-of-care diagnostics of urinary tract infections has also been demonstrated. Using uropathogenic E. coli clinical isolates as model systems, we demonstrate that "on-chip" ACEF-induced sample preparation can improve the sensor performance without complicated system integration strategy and presents a pathway for implementing truly lab on a chip, instead of chip in a lab. Finally an integrated chip approach has been proposed for transforming 26 electrochemical sensing system from laboratory research into point-of-care diagnostics with multiple microelectrodes.
590 $a School code: 0009.
650 4 $a Engineering, Mechanical. $3 845387
690 $a 0548
710 2 $a The University of Arizona. $b Mechanical Engineering. $3 845457
773 0 $t Dissertation Abstracts International $g 72-12B.
790 1 0 $a Wong, Pak Kin, $e advisor
790 1 0 $a Chan, Cho Lik $e committee member
790 1 0 $a Zohar, Yitshak $e committee member
790 $a 0009
791 $a Ph.D.
792 $a 2011
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