國立虎尾科技大學 |

Three Dimensional Bioprinting and Micro/Nano-particle Integration for Complex, Gradient Osteochondral Tissue Engineering Scaffolds.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Three Dimensional Bioprinting and Micro/Nano-particle Integration for Complex, Gradient Osteochondral Tissue Engineering Scaffolds./
Author:	Nowicki, Margaret A.
Description:	1 online resource (139 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 78-08(E), Section: B.
Contained By:	Dissertation Abstracts International78-08B(E).
Subject:	Mechanical engineering. -
Online resource:	click for full text (PQDT)
ISBN:	9781369684704

Three Dimensional Bioprinting and Micro/Nano-particle Integration for Complex, Gradient Osteochondral Tissue Engineering Scaffolds.
Nowicki, Margaret A.

Three Dimensional Bioprinting and Micro/Nano-particle Integration for Complex, Gradient Osteochondral Tissue Engineering Scaffolds. - 1 online resource (139 pages)

Source: Dissertation Abstracts International, Volume: 78-08(E), Section: B.

Thesis (Ph.D.)

Includes bibliographical references

Osteochondral tissue has a complex graded structure where biological, physiological, and mechanical properties vary significantly over the full thickness spanning the subchondral bone region beneath the joint surface to the hyaline cartilage region at the joint surface. This presents a significant challenge for tissue-engineered structures addressing osteochondral defects. 3D bioprinters, together with novel biomaterials and innovative fabrication techniques, present a unique solution to this problem. The objective of this body of research is to use FDM-based 3D bioprinting, innovative biomaterials, appropriate growth factors, and innovative casting techniques for improved bone marrow human mesenchymal stem cell (hMSC) adhesion, growth, and osteochondral differentiation. FDM parameters can be tuned through computer aided design and computer numerical control software to manipulate scaffold geometries in ways that are beneficial to mechanical performance without hindering cellular behavior. Additionally, the ability to manipulate 3D printed scaffolds increases further through our innovative casting procedure which facilitates the inclusion of nanoparticles with biochemical factors to further elicit desired hMSC differentiation. In all studies the mechanical and biological impacts of the scaffolds were compared and evaluated to determine the benefits of each physical manipulation. The results indicate that both mechanical properties and cell performance can be easily manipulated through the investment casting process to achieve a spatially appropriate osteogenic and chondrogenic response in engineered osteochondral scaffolds.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9781369684704Subjects--Topical Terms:

557493
Mechanical engineering.
Index Terms--Genre/Form:

554714
Electronic books.

Three Dimensional Bioprinting and Micro/Nano-particle Integration for Complex, Gradient Osteochondral Tissue Engineering Scaffolds.
LDR:02997ntm a2200337Ki 4500 001 908668
005 20180330125240.5
006 m o u
007 cr mn||||a|a||
008 190606s2017 xx obm 000 0 eng d
020 $a 9781369684704
035 $a (MiAaPQ)AAI10264231
035 $a (MiAaPQ)gwu:13559
035 $a AAI10264231
040 $a MiAaPQ $b eng $c MiAaPQ
099 $a TUL $f hyy $c available through World Wide Web
100 1 $a Nowicki, Margaret A. $3 1178912
245 1 0 $a Three Dimensional Bioprinting and Micro/Nano-particle Integration for Complex, Gradient Osteochondral Tissue Engineering Scaffolds.
264 0 $c 2017
300 $a 1 online resource (139 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertation Abstracts International, Volume: 78-08(E), Section: B.
500 $a Advisers: Michael Plesniak; Lijie Grace Zhang.
502 $a Thesis (Ph.D.) $c The George Washington University $d 2017.
504 $a Includes bibliographical references
520 $a Osteochondral tissue has a complex graded structure where biological, physiological, and mechanical properties vary significantly over the full thickness spanning the subchondral bone region beneath the joint surface to the hyaline cartilage region at the joint surface. This presents a significant challenge for tissue-engineered structures addressing osteochondral defects. 3D bioprinters, together with novel biomaterials and innovative fabrication techniques, present a unique solution to this problem. The objective of this body of research is to use FDM-based 3D bioprinting, innovative biomaterials, appropriate growth factors, and innovative casting techniques for improved bone marrow human mesenchymal stem cell (hMSC) adhesion, growth, and osteochondral differentiation. FDM parameters can be tuned through computer aided design and computer numerical control software to manipulate scaffold geometries in ways that are beneficial to mechanical performance without hindering cellular behavior. Additionally, the ability to manipulate 3D printed scaffolds increases further through our innovative casting procedure which facilitates the inclusion of nanoparticles with biochemical factors to further elicit desired hMSC differentiation. In all studies the mechanical and biological impacts of the scaffolds were compared and evaluated to determine the benefits of each physical manipulation. The results indicate that both mechanical properties and cell performance can be easily manipulated through the investment casting process to achieve a spatially appropriate osteogenic and chondrogenic response in engineered osteochondral scaffolds.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Mechanical engineering. $3 557493
655 7 $a Electronic books. $2 local $3 554714
690 $a 0548
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a The George Washington University. $b Mechanical and Aerospace Engineering. $3 1178913
773 0 $t Dissertation Abstracts International $g 78-08B(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10264231 $z click for full text (PQDT)