國立虎尾科技大學 |

Development of electrochemical microsensors to use in bioelectrochemical systems.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Development of electrochemical microsensors to use in bioelectrochemical systems./
Author:	Atci, Erhan.
Description:	1 online resource (195 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 78-01(E), Section: B.
Contained By:	Dissertation Abstracts International78-01B(E).
Subject:	Chemical engineering. -
Online resource:	click for full text (PQDT)
ISBN:	9781339950198

Development of electrochemical microsensors to use in bioelectrochemical systems.
Atci, Erhan.

Development of electrochemical microsensors to use in bioelectrochemical systems. - 1 online resource (195 pages)

Source: Dissertation Abstracts International, Volume: 78-01(E), Section: B.

Thesis (Ph.D.)

Includes bibliographical references

The objective of this dissertation was to develop electrochemical microsensors to quantify microscale gradients within biofilms grown in bioelectrochemical systems (BESs). In BESs, generally three electrodes are used: an anode accepting electrons from the biofilms, a cathode donating electrons to biofilms or generating biocides, and a reference electrode. The anodic biofilms are grown for energy and biochemical production. In addition, some BESs are used to generate biocides at the cathodes. When biofilms are used for energy generation, the electron donor gradients play a critical role and can control electron transfer rates as well as energy generation. On the other hand, when the cathodes of BESs are used to generate hydrogen peroxide to prevent biofilm growth, its concentration is critically needed to understand the applicability of this technology. Currently, there is no electrochemical microsensors which can operate in BESs. This dissertation focuses on the development and applications of electrochemical microsensors to quantify electron donor and electron acceptor concentrations on electrodes employed in BESs. A hydrogen peroxide microelectrode was developed to measure hydrogen peroxide concentrations on cathodically polarized surfaces. We found that the maximum hydrogen peroxide concentration on cathodically polarized surfaces was ~25 microM which was enough to eliminate biofilms. This discovery allowed us to develop an electrochemical technology to eliminate unwanted biofilms on cathodically polarized surfaces. Then, we developed two microbiosensors (acetate and fumarate) which were operated based on similar principles. The working principle of the acetate microbiosensor was based on the correlation between the acetate concentration in the vicinity of the microbiosensor and the current generated during acetate oxidation by Geobacter sulfurreducens acting as a transducer in the microbiosensor. When we used it in anodic biofilms, we found that the acetate was limiting in the biofilm and the bottom of the biofilm was serving as a scaffold for the top layer. The fumarate microbiosensor used G. sulfurreducens acting as a transducer for the cathodic reactions. When we used fumarate microbiosensor in cathodic biofilms, we found that fumarate can be reduced while an electrode is used as an electron donor and the fumarate reduction was potential-dependent in the biofilm.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9781339950198Subjects--Topical Terms:

555952
Chemical engineering.
Index Terms--Genre/Form:

554714
Electronic books.

Development of electrochemical microsensors to use in bioelectrochemical systems.
LDR:03702ntm a2200349Ki 4500 001 910692
005 20180517112607.5
006 m o u
007 cr mn||||a|a||
008 190606s2016 xx obm 000 0 eng d
020 $a 9781339950198
035 $a (MiAaPQ)AAI10139534
035 $a (MiAaPQ)wsu:11602
035 $a AAI10139534
040 $a MiAaPQ $b eng $c MiAaPQ
099 $a TUL $f hyy $c available through World Wide Web
100 1 $a Atci, Erhan. $3 1182116
245 1 0 $a Development of electrochemical microsensors to use in bioelectrochemical systems.
264 0 $c 2016
300 $a 1 online resource (195 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertation Abstracts International, Volume: 78-01(E), Section: B.
500 $a Adviser: Haluk Beyenal.
502 $a Thesis (Ph.D.) $c Washington State University $d 2016.
504 $a Includes bibliographical references
520 $a The objective of this dissertation was to develop electrochemical microsensors to quantify microscale gradients within biofilms grown in bioelectrochemical systems (BESs). In BESs, generally three electrodes are used: an anode accepting electrons from the biofilms, a cathode donating electrons to biofilms or generating biocides, and a reference electrode. The anodic biofilms are grown for energy and biochemical production. In addition, some BESs are used to generate biocides at the cathodes. When biofilms are used for energy generation, the electron donor gradients play a critical role and can control electron transfer rates as well as energy generation. On the other hand, when the cathodes of BESs are used to generate hydrogen peroxide to prevent biofilm growth, its concentration is critically needed to understand the applicability of this technology. Currently, there is no electrochemical microsensors which can operate in BESs. This dissertation focuses on the development and applications of electrochemical microsensors to quantify electron donor and electron acceptor concentrations on electrodes employed in BESs. A hydrogen peroxide microelectrode was developed to measure hydrogen peroxide concentrations on cathodically polarized surfaces. We found that the maximum hydrogen peroxide concentration on cathodically polarized surfaces was ~25 microM which was enough to eliminate biofilms. This discovery allowed us to develop an electrochemical technology to eliminate unwanted biofilms on cathodically polarized surfaces. Then, we developed two microbiosensors (acetate and fumarate) which were operated based on similar principles. The working principle of the acetate microbiosensor was based on the correlation between the acetate concentration in the vicinity of the microbiosensor and the current generated during acetate oxidation by Geobacter sulfurreducens acting as a transducer in the microbiosensor. When we used it in anodic biofilms, we found that the acetate was limiting in the biofilm and the bottom of the biofilm was serving as a scaffold for the top layer. The fumarate microbiosensor used G. sulfurreducens acting as a transducer for the cathodic reactions. When we used fumarate microbiosensor in cathodic biofilms, we found that fumarate can be reduced while an electrode is used as an electron donor and the fumarate reduction was potential-dependent in the biofilm.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Chemical engineering. $3 555952
650 4 $a Analytical chemistry. $3 1182118
655 7 $a Electronic books. $2 local $3 554714
690 $a 0542
690 $a 0486
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Washington State University. $b Chemical Engineering. $3 1182117
773 0 $t Dissertation Abstracts International $g 78-01B(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10139534 $z click for full text (PQDT)