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MUTAGENESIS OF POLYCYCLIC AROMATIC HYDROCARBONS AND N-HETEROCYCLIC AROMATICS IN THE CHINESE HAMSTER DPI-3 EPITHELIAL CELL LINE (NORMAL).

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	MUTAGENESIS OF POLYCYCLIC AROMATIC HYDROCARBONS AND N-HETEROCYCLIC AROMATICS IN THE CHINESE HAMSTER DPI-3 EPITHELIAL CELL LINE (NORMAL)./
Author:	DOOLEY, JOHN FRANKLIN, III.
Description:	1 online resource (233 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 45-08, Section: B, page: 2483.
Subject:	Environmental science. -
Online resource:	click for full text (PQDT)

MUTAGENESIS OF POLYCYCLIC AROMATIC HYDROCARBONS AND N-HETEROCYCLIC AROMATICS IN THE CHINESE HAMSTER DPI-3 EPITHELIAL CELL LINE (NORMAL).
DOOLEY, JOHN FRANKLIN, III.

MUTAGENESIS OF POLYCYCLIC AROMATIC HYDROCARBONS AND N-HETEROCYCLIC AROMATICS IN THE CHINESE HAMSTER DPI-3 EPITHELIAL CELL LINE (NORMAL). - 1 online resource (233 pages)

Source: Dissertation Abstracts International, Volume: 45-08, Section: B, page: 2483.

Thesis (Ph.D.)--University of Cincinnati, 1984.

Includes bibliographical references

Previous studies have described the clonal isolation of the DPI-3 non-tumorigenic epithelial cell line from a primary explant of a Chinese hamster female embryo. The purpose of this study was to further characterize the chromosomal stability, non-tumorigenic nature and epithelial origin of the DPI-3 cell line and to determine the mutagenic response of the DPI-3 cells to a series of carcinogenic and non-carcinogenic N-heterocyclic aromatic (N-HA) and polycyclic aromatic hydrocarbons (PAH).

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

Subjects--Topical Terms:

1179128
Environmental science.
Index Terms--Genre/Form:

554714
Electronic books.

MUTAGENESIS OF POLYCYCLIC AROMATIC HYDROCARBONS AND N-HETEROCYCLIC AROMATICS IN THE CHINESE HAMSTER DPI-3 EPITHELIAL CELL LINE (NORMAL).
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245 1 0 $a MUTAGENESIS OF POLYCYCLIC AROMATIC HYDROCARBONS AND N-HETEROCYCLIC AROMATICS IN THE CHINESE HAMSTER DPI-3 EPITHELIAL CELL LINE (NORMAL).
264 0 $c 1984
300 $a 1 online resource (233 pages)
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500 $a Source: Dissertation Abstracts International, Volume: 45-08, Section: B, page: 2483.
502 $a Thesis (Ph.D.)--University of Cincinnati, 1984.
504 $a Includes bibliographical references
520 $a Previous studies have described the clonal isolation of the DPI-3 non-tumorigenic epithelial cell line from a primary explant of a Chinese hamster female embryo. The purpose of this study was to further characterize the chromosomal stability, non-tumorigenic nature and epithelial origin of the DPI-3 cell line and to determine the mutagenic response of the DPI-3 cells to a series of carcinogenic and non-carcinogenic N-heterocyclic aromatic (N-HA) and polycyclic aromatic hydrocarbons (PAH).
520 $a Karyotypic analyses demonstrated that, while the modal chromosome number increased with time in culture, the chromosomal constitution of the DPI-3 cells is relatively stable and near-diploid. Anchorage-independence and tumorigenicity studies demonstrated that late passage DPI-3 cells do not form colonies in soft agar or tumors in syngeneic recipients. Ultrastructural studies revealed the presence of desmosomes and tight junctions between the apical-lateral surfaces of adjacent DPI-3 cells. These findings demonstrate the chromosomal stability of the DPI-3 cells and confirm their non-tumorigenic nature and epithelial origin.
520 $a Initial mutagenesis experiments were conducted with methylazoxymethanol acetate (MAM) to determine the optimum selection conditions and phenotypic expression time for quantitating mutation induction at the hypoxanthine-guanine phosphoribosyl transferase locus. MAM produced a dose-dependent increase in the 6-thioguanine resistant (6-TG('r)) mutant frequency of DPI-3 cells under optimum selection conditions. Optimal expression of MAM-induced 6-TG('r) mutants occurred after 8 days of expression and remained stable throughout 16 days of expression. In subsequent mutagenesis experiments with several carcinogenic N-HA and PAH, 7,12-dimethylbenz(a)anthracene {DMBA} and dibenzo(c,g)carbazole {DB(c,g)C} were mutagenic to DPI-3 cells, whereas benzo(a)pyrene {B(a)P} and dibenz(a,j)acridine {DB(a,j)Ac} were not. These results demonstrate that although DPI-3 cells metabolize certain N-HA and PAH to mutagenic metabolites, the endogenous metabolism of DPI-3 cells needs to be supplemented for studying the mutagenicity of these two classes of carcinogens.
520 $a Metabolism and cell-mediated mutagenesis experiments with X-irradiated and non-irradiated Syrian hamster embryo (SHE) cells demonstrated that non-irradiated SHE cells could be used to supplement the endogenous metabolism of the DPI-3 cells. . . . (Author's abstract exceeds stipulated maximum length. Discontinued here with permission of author.) UMI.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Environmental science. $3 1179128
655 7 $a Electronic books. $2 local $3 554714
690 $a 0768
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of Cincinnati. $3 1187930
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8420869 $z click for full text (PQDT)