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Using Comparative Genomics in Saccharomyces cerevisiae to Engineer Lignocellulosic Hydrolysate Tolerance.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Using Comparative Genomics in Saccharomyces cerevisiae to Engineer Lignocellulosic Hydrolysate Tolerance./
Author:	Sardi, Maria Isabel.
Description:	1 online resource (195 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 78-12(E), Section: B.
Subject:	Microbiology. -
Online resource:	click for full text (PQDT)
ISBN:	9780355098358

Using Comparative Genomics in Saccharomyces cerevisiae to Engineer Lignocellulosic Hydrolysate Tolerance.
Sardi, Maria Isabel.

Using Comparative Genomics in Saccharomyces cerevisiae to Engineer Lignocellulosic Hydrolysate Tolerance. - 1 online resource (195 pages)

Source: Dissertation Abstracts International, Volume: 78-12(E), Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2017.

Includes bibliographical references

The increased interest in alternative fuels is driving the development of more efficient and economical production of biofuels. This requires the use of non-food based plant biomass to produce advanced biofuels such as butanol and isobutanol. A major challenge of implementing this new energy source is that the chemically treated plant material, known as lignocellulosic hydrolysate, contains a variety of toxic compounds that affect fermenting microbes, decreasing the economic efficiency of lignocellulosic biofuel production. In addition, butanol and isobutanol are toxic even at small concentrations, making end product toxicity a significant limiting factor. In this thesis, we report the use of multiple genomic strategies to identify mechanisms of toxicity and tolerance that can be then use to engineer tolerance into industrially relevant microbes. First, by comparing and contrasting the transcriptional responses of tolerant and sensitive Saccharomyces cerevisiae strains exposed to these stresses, we identified primary toxin targets and their effects on cellular physiology. Second, we explored genetic differences among strains to performed a genome wide association study that identified genetic variants correlated with tolerance to plant hydrolysate. By applying multiple genomic methods and integrating the results, we identified strategies for improving tolerance to the stresses found in the production of advanced biofuels from plant hydrolysate and identified large effects of genetic background on phenotypic outcome, which highlights challenges in predicting the most beneficial engineering strategies for each specific strain.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355098358Subjects--Topical Terms:

591510
Microbiology.
Index Terms--Genre/Form:

554714
Electronic books.

Using Comparative Genomics in Saccharomyces cerevisiae to Engineer Lignocellulosic Hydrolysate Tolerance.
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245 1 0 $a Using Comparative Genomics in Saccharomyces cerevisiae to Engineer Lignocellulosic Hydrolysate Tolerance.
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300 $a 1 online resource (195 pages)
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500 $a Source: Dissertation Abstracts International, Volume: 78-12(E), Section: B.
500 $a Adviser: Audrey P. Gasch.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2017.
504 $a Includes bibliographical references
520 $a The increased interest in alternative fuels is driving the development of more efficient and economical production of biofuels. This requires the use of non-food based plant biomass to produce advanced biofuels such as butanol and isobutanol. A major challenge of implementing this new energy source is that the chemically treated plant material, known as lignocellulosic hydrolysate, contains a variety of toxic compounds that affect fermenting microbes, decreasing the economic efficiency of lignocellulosic biofuel production. In addition, butanol and isobutanol are toxic even at small concentrations, making end product toxicity a significant limiting factor. In this thesis, we report the use of multiple genomic strategies to identify mechanisms of toxicity and tolerance that can be then use to engineer tolerance into industrially relevant microbes. First, by comparing and contrasting the transcriptional responses of tolerant and sensitive Saccharomyces cerevisiae strains exposed to these stresses, we identified primary toxin targets and their effects on cellular physiology. Second, we explored genetic differences among strains to performed a genome wide association study that identified genetic variants correlated with tolerance to plant hydrolysate. By applying multiple genomic methods and integrating the results, we identified strategies for improving tolerance to the stresses found in the production of advanced biofuels from plant hydrolysate and identified large effects of genetic background on phenotypic outcome, which highlights challenges in predicting the most beneficial engineering strategies for each specific strain.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Microbiology. $3 591510
650 4 $a Genetics. $3 578972
650 4 $a Bioinformatics. $3 583857
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690 $a 0369
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710 2 $a The University of Wisconsin - Madison. $b Microbiology ALS. $3 1148563
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