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Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants./
Author:	Diamos, Andy.
Description:	1 online resource (272 pages)
Notes:	Source: Dissertation Abstracts International, Volume: 79-04(E), Section: B.
Subject:	Molecular biology. -
Online resource:	click for full text (PQDT)
ISBN:	9780355512892

Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants.
Diamos, Andy.

Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants. - 1 online resource (272 pages)

Source: Dissertation Abstracts International, Volume: 79-04(E), Section: B.

Thesis (Ph.D.)--Arizona State University, 2017.

Includes bibliographical references

Plants are a promising upcoming platform for production of vaccine components and other desirable pharmaceutical proteins that can only, at present, be made in living systems. The unique soil microbe Agrobacterium tumefaciens can transfer DNA to plants very efficiently, essentially turning plants into factories capable of producing virtually any gene. While genetically modified bacteria have historically been used for producing useful biopharmaceuticals like human insulin, plants can assemble much more complicated proteins, like human antibodies, that bacterial systems cannot. As plants do not harbor human pathogens, they are also safer alternatives than animal cell cultures. Additionally, plants can be grown very cheaply, in massive quantities.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355512892Subjects--Topical Terms:

583443
Molecular biology.
Index Terms--Genre/Form:

554714
Electronic books.

Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants.
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100 1 $a Diamos, Andy. $3 1148662
245 1 0 $a Optimization of a Viral System to Produce Vaccines and other Biopharmaceuticals in Plants.
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300 $a 1 online resource (272 pages)
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500 $a Source: Dissertation Abstracts International, Volume: 79-04(E), Section: B.
500 $a Adviser: Hugh S. Mason.
502 $a Thesis (Ph.D.)--Arizona State University, 2017.
504 $a Includes bibliographical references
520 $a Plants are a promising upcoming platform for production of vaccine components and other desirable pharmaceutical proteins that can only, at present, be made in living systems. The unique soil microbe Agrobacterium tumefaciens can transfer DNA to plants very efficiently, essentially turning plants into factories capable of producing virtually any gene. While genetically modified bacteria have historically been used for producing useful biopharmaceuticals like human insulin, plants can assemble much more complicated proteins, like human antibodies, that bacterial systems cannot. As plants do not harbor human pathogens, they are also safer alternatives than animal cell cultures. Additionally, plants can be grown very cheaply, in massive quantities.
520 $a In my research, I have studied the genetic mechanisms that underlie gene expression, in order to improve plant-based biopharmaceutical production. To do this, inspiration was drawn from naturally-occurring gene regulatory mechanisms, especially those from plant viruses, which have evolved mechanisms to co-opt the plant cellular machinery to produce high levels of viral proteins. By testing, modifying, and combining genetic elements from diverse sources, an optimized expression system has been developed that allows very rapid production of vaccine components, monoclonal antibodies, and other biopharmaceuticals. To improve target gene expression while maintaining the health and function of the plants, I identified, studied, and modified 5' untranslated regions, combined gene terminators, and a nuclear matrix attachment region. The replication mechanisms of a plant geminivirus were also studied, which lead to additional strategies to produce more toxic biopharmaceutical proteins. Finally, the mechanisms employed by a geminivirus to spread between cells were investigated. It was demonstrated that these movement mechanisms can be functionally transplanted into a separate genus of geminivirus, allowing modified virus-based gene expression vectors to be spread between neighboring plant cells. Additionally, my work helps shed light on the basic genetic mechanisms employed by all living organisms to control gene expression.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Molecular biology. $3 583443
650 4 $a Genetics. $3 578972
650 4 $a Virology. $3 644995
655 7 $a Electronic books. $2 local $3 554714
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690 $a 0369
690 $a 0720
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a Arizona State University. $b Microbiology. $3 1148663
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10681475 $z click for full text (PQDT)