國立虎尾科技大學 |

Development and Characterisation of Nano Fiber Based Skin Equivalent Model.

Record Type:	Language materials, manuscript : Monograph/item
Title/Author:	Development and Characterisation of Nano Fiber Based Skin Equivalent Model./
Author:	Nomhwange, Hembafan.
Description:	1 online resource (60 pages)
Notes:	Source: Masters Abstracts International, Volume: 57-05.
Contained By:	Masters Abstracts International57-05(E).
Subject:	Biomedical engineering. -
Online resource:	click for full text (PQDT)
ISBN:	9780355940909

Development and Characterisation of Nano Fiber Based Skin Equivalent Model.
Nomhwange, Hembafan.

Development and Characterisation of Nano Fiber Based Skin Equivalent Model. - 1 online resource (60 pages)

Source: Masters Abstracts International, Volume: 57-05.

Thesis (M.S.)--University of Central Oklahoma, 2018.

Includes bibliographical references

Over the past three decades, significant progress has been made in the area of skin tissue engineering using different types of scaffolds such as non-cell based grafts and cell based grafts. The skin consists of the epidermis, dermis, and hypodermis, the dermis is the thickest layer and so far challenges in skin tissue engineering has been difficulty to restore the dermis. Fibro blasts secrete collagen and elastin which gives mechanical strength and elasticity to the skin which is the main function of the dermis. The aim of this study. is to develop a skin graft model that can mimic the structure and function of the dermis by using Electrospun Nano fiber coating to design polyethylene Glycol Diacrylate(PEGDA) poly e-caprolactone (PCL) scaffolds, these 3 different scaffolds namely (PEGDA) poly e-caprolactone (PCL), Sodium Alginate PCL and Collagen PCL were cultured using fibro blast cells. Analysis of the development and characterization of these fiber based skin grafts was done by checking the architectural integrity by degradation analysis, cell viability through proliferation of cells, cell differentiation, and cell addition. Furthermore, scanning electron microscopy SEM (histology section) of PEGDA PCL, sodium alginate PCL and collagen PCL without cells was used to see the adhesiveness of PCL fibers with the different scaffold. Also, this study involved morphometric analysis through Wettability of PEGDA PCL, Sodium Alginate PCL, and Collagen PCL to see its ability to absorb water. Cytotoxicity of cells showed that cells sticks to the bottom of samples. During this study, it was seen that Collagen PCL and Sodium Alginate PCL showed better characteristics regarding being compatible with the normal skin if used as a skin graft.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2018

Mode of access: World Wide Web

ISBN: 9780355940909Subjects--Topical Terms:

588770
Biomedical engineering.
Index Terms--Genre/Form:

554714
Electronic books.

Development and Characterisation of Nano Fiber Based Skin Equivalent Model.
LDR:02965ntm a2200337Ki 4500 001 918409
005 20181114145236.5
006 m o u
007 cr mn||||a|a||
008 190606s2018 xx obm 000 0 eng d
020 $a 9780355940909
035 $a (MiAaPQ)AAI10815278
035 $a (MiAaPQ)uco:10464
035 $a AAI10815278
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Nomhwange, Hembafan. $3 1192726
245 1 0 $a Development and Characterisation of Nano Fiber Based Skin Equivalent Model.
264 0 $c 2018
300 $a 1 online resource (60 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 57-05.
500 $a Adviser: Morshed Khandaker.
502 $a Thesis (M.S.)--University of Central Oklahoma, 2018.
504 $a Includes bibliographical references
520 $a Over the past three decades, significant progress has been made in the area of skin tissue engineering using different types of scaffolds such as non-cell based grafts and cell based grafts. The skin consists of the epidermis, dermis, and hypodermis, the dermis is the thickest layer and so far challenges in skin tissue engineering has been difficulty to restore the dermis. Fibro blasts secrete collagen and elastin which gives mechanical strength and elasticity to the skin which is the main function of the dermis. The aim of this study. is to develop a skin graft model that can mimic the structure and function of the dermis by using Electrospun Nano fiber coating to design polyethylene Glycol Diacrylate(PEGDA) poly e-caprolactone (PCL) scaffolds, these 3 different scaffolds namely (PEGDA) poly e-caprolactone (PCL), Sodium Alginate PCL and Collagen PCL were cultured using fibro blast cells. Analysis of the development and characterization of these fiber based skin grafts was done by checking the architectural integrity by degradation analysis, cell viability through proliferation of cells, cell differentiation, and cell addition. Furthermore, scanning electron microscopy SEM (histology section) of PEGDA PCL, sodium alginate PCL and collagen PCL without cells was used to see the adhesiveness of PCL fibers with the different scaffold. Also, this study involved morphometric analysis through Wettability of PEGDA PCL, Sodium Alginate PCL, and Collagen PCL to see its ability to absorb water. Cytotoxicity of cells showed that cells sticks to the bottom of samples. During this study, it was seen that Collagen PCL and Sodium Alginate PCL showed better characteristics regarding being compatible with the normal skin if used as a skin graft.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2018
538 $a Mode of access: World Wide Web
650 4 $a Biomedical engineering. $3 588770
650 4 $a Nanotechnology. $3 557660
655 7 $a Electronic books. $2 local $3 554714
690 $a 0541
690 $a 0652
710 2 $a ProQuest Information and Learning Co. $3 1178819
710 2 $a University of Central Oklahoma. $b Engineering & Physics. $3 1187594
773 0 $t Masters Abstracts International $g 57-05(E).
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10815278 $z click for full text (PQDT)