國立虎尾科技大學 |

Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis/ by Bin Zhuang.
Author:	Zhuang, Bin.
Description:	XXIII, 114 p. 58 illus., 44 illus. in color.online resource. :
Contained By:	Springer Nature eBook
Subject:	Biomedical engineering. -
Online resource:	https://doi.org/10.1007/978-981-10-4753-4
ISBN:	9789811047534

Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis
Zhuang, Bin.

Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis[electronic resource] /by Bin Zhuang. - 1st ed. 2018. - XXIII, 114 p. 58 illus., 44 illus. in color.online resource. - Springer Theses, Recognizing Outstanding Ph.D. Research,2190-5053. - Springer Theses, Recognizing Outstanding Ph.D. Research,.

Introduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work.

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

ISBN: 9789811047534

Standard No.: 10.1007/978-981-10-4753-4doiSubjects--Topical Terms:

588770
Biomedical engineering.

LC Class. No.: R856-857

Dewey Class. No.: 610.28

Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis
LDR:02886nam a22004335i 4500 001 986986
003 DE-He213
005 20200703155037.0
007 cr nn 008mamaa
008 201225s2018 si | s |||| 0|eng d
020 $a 9789811047534 $9 978-981-10-4753-4
024 7 $a 10.1007/978-981-10-4753-4 $2 doi
035 $a 978-981-10-4753-4
050 4 $a R856-857
050 4 $a HC79.E5
050 4 $a GE220
072 7 $a MQW $2 bicssc
072 7 $a TEC059000 $2 bisacsh
072 7 $a MQW $2 thema
082 0 4 $a 610.28 $2 23
100 1 $a Zhuang, Bin. $e author. $4 aut $4 http://id.loc.gov/vocabulary/relators/aut $3 1279486
245 1 0 $a Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis $h [electronic resource] / $c by Bin Zhuang.
250 $a 1st ed. 2018.
264 1 $a Singapore : $b Springer Singapore : $b Imprint: Springer, $c 2018.
300 $a XXIII, 114 p. 58 illus., 44 illus. in color. $b online resource.
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
347 $a text file $b PDF $2 rda
490 1 $a Springer Theses, Recognizing Outstanding Ph.D. Research, $x 2190-5053
505 0 $a Introduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work.
520 $a This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.
650 0 $a Biomedical engineering. $3 588770
650 0 $a Genetic engineering. $3 592227
650 0 $a Nucleic acids. $3 788506
650 1 4 $a Biomedical Engineering and Bioengineering. $3 1211019
650 2 4 $a Genetic Engineering. $3 559617
650 2 4 $a Nucleic Acid Chemistry. $3 716485
710 2 $a SpringerLink (Online service) $3 593884
773 0 $t Springer Nature eBook
776 0 8 $i Printed edition: $z 9789811047527
776 0 8 $i Printed edition: $z 9789811047541
776 0 8 $i Printed edition: $z 9789811352201
830 0 $a Springer Theses, Recognizing Outstanding Ph.D. Research, $x 2190-5053 $3 1253569
856 4 0 $u https://doi.org/10.1007/978-981-10-4753-4
912 $a ZDB-2-ENG
912 $a ZDB-2-SXE
950 $a Engineering (SpringerNature-11647)
950 $a Engineering (R0) (SpringerNature-43712)